School of Chemistry and Chemical Engineering, Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University, Nanjing 211189, P. R. China.
Langmuir. 2021 Dec 7;37(48):14167-14176. doi: 10.1021/acs.langmuir.1c02433. Epub 2021 Nov 28.
Magnetic materials perform well in the purification of histidine-rich proteins (His-proteins). In this work, a facile fabrication of yolk-shell magnetic FeO@NiSiO/Ni microspheres for the efficient purification of His-proteins has been reported. Yolk-shell FeO@NiSiO microspheres were prepared via hydrothermal reaction. Then Ni nanoparticles (NPs) were loaded on FeO@NiSiO microspheres after the adsorption and in situ reduction of nickel acetylacetonate. The yolk-shell FeO@NiSiO/Ni microspheres had a hierarchical flower-like structure and large cavities. The size of the cavity depended on the reaction time. This indicated that the microspheres had a large specific surface area for loading of more Ni NPs, which was crucial to the high His-protein adsorption capacity of FeO@NiSiO/Ni microspheres. FeO@NiSiO/Ni microspheres had a high adsorption capacity for bovine hemoglobin (BHb, 2822 mg/g), which was better than the values of other His-protein adsorbents. FeO@NiSiO/Ni microspheres still had a high BHb separation efficiency after seven separation cycles, indicating its good reusability and stability. Therefore, the as-prepared bifunctional yolk-shell FeO@NiSiO/Ni microspheres exhibited great practical application value for His-protein purification.
磁性材料在组氨酸丰富的蛋白质（His-蛋白）的纯化中表现良好。在这项工作中，报道了一种简便的蛋黄壳型磁性 FeO@NiSiO/Ni 微球的制备方法，用于高效纯化 His-蛋白。通过水热反应制备了蛋黄壳型 FeO@NiSiO 微球。然后，在乙酰丙酮镍吸附和原位还原后，将 Ni 纳米颗粒（NPs）负载在 FeO@NiSiO 微球上。蛋黄壳型 FeO@NiSiO/Ni 微球具有分级花状结构和大空腔。空腔的大小取决于反应时间。这表明微球具有较大的比表面积，可负载更多的 Ni NPs，这对 FeO@NiSiO/Ni 微球的高 His-蛋白吸附容量至关重要。FeO@NiSiO/Ni 微球对牛血红蛋白（BHb，2822mg/g）具有高吸附容量，优于其他 His-蛋白吸附剂的值。FeO@NiSiO/Ni 微球在经过七个分离循环后仍具有高的 BHb 分离效率，表明其具有良好的可重复使用性和稳定性。因此，所制备的双功能蛋黄壳型 FeO@NiSiO/Ni 微球在 His-蛋白纯化方面具有很大的实际应用价值。
