Ćućuz Veljko, Cvejić Jelena, Torović Ljilja, Gojković-Bukarica Ljiljana, Acevska Jelena, Dimitrovska Aneta, Aldawoud Turki M S, Galanakis Charis M
Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, Serbia.
Faculty of Medicine, Institute of Pharmacology, Clinical Pharmacology and Toxicology, University of Belgrade, Doktora Subotića 8, Belgrade, Serbia.
J Food Sci Technol. 2022 Jul;59(7):2913-2924. doi: 10.1007/s13197-021-05317-9. Epub 2021 Nov 22.
Grape processing by-products (particularly grape pomace) are known to contain high amounts of phenolic compounds. To improve the extraction of phenols from this by-product, it is necessary to develop a method and set and model optimal conditions for their extraction. By applying the design of experiments (DoE) approach, optimal experimental factors of Ultrasound-assisted extraction (USAE) were determined to obtain grape pomace extracts with a satisfactory yield of phenols anthocyanins, as well as extracts with high antioxidant capacity using reagents approved in the food industry. Initial method optimization covered two experimental factors: solvent concentration and the weight ratio of the sample and solvent using fixed USAE conditions from literature. For the final method optimization, the three investigated experimental factors were: pH value, the temperature of extraction, and extraction time. The optimal experimental conditions for the development of the method were 55% ethanol, sample/solvent ratio 1:40, pH 4.5, T 55 °C, and 30 min. Depending on the primary goal of the extraction process (the antioxidant activity, total phenolic content, content of individual phenols, or content of individual anthocyanins), these parameters can easily be modified to obtain the desired recovery.
The online version contains supplementary material available at 10.1007/s13197-021-05317-9.
葡萄加工副产物（特别是葡萄皮渣）已知含有大量酚类化合物。为了提高从该副产物中提取酚类物质的效率，有必要开发一种方法并设定和建立其提取的最佳条件模型。通过应用实验设计（DoE）方法，确定了超声辅助提取（USAE）的最佳实验因素，以获得具有令人满意的酚类、花青素产量的葡萄皮渣提取物，以及使用食品工业中批准的试剂具有高抗氧化能力的提取物。初始方法优化涵盖两个实验因素：溶剂浓度以及使用文献中固定的USAE条件下样品与溶剂的重量比。对于最终方法优化，研究的三个实验因素为：pH值、提取温度和提取时间。该方法开发的最佳实验条件为55%乙醇、样品/溶剂比1:40、pH 4.5、温度55°C和30分钟。根据提取过程的主要目标（抗氧化活性、总酚含量、单个酚类物质的含量或单个花青素的含量），这些参数可以很容易地修改以获得所需的回收率。
在线版本包含可在10.1007/s13197-
