Le Peter B, Chen Fang, Myung David
Department of Ophthalmology, Stanford University School of Medicine, CA, USA.
VA Palo Alto Health Care System, Palo Alto, CA, USA.
J Ophthalmic Vis Res. 2021 Oct 25;16(4):544-551. doi: 10.18502/jovr.v16i4.9743. eCollection 2021 Oct-Dec.
Creating controllable, reproducible keratectomy wounds in rodent corneas can be a challenge due to their small size, thickness, and the lack of usual tools available for human eyes such as a vacuum trephine. The purpose of this work is to provide a consistent, reproducible corneal stromal defect in rats using a simple, economical, and customized inner-stopper guarded trephine.
The inner-stopper guarded trephine is used to induce a circular wound in rat corneas. After trephination, the corneal flap can be removed by manual dissection using a blunt spatula. We used optical coherence topography (OCT) to measure the defect wound depth induced in rat eyes.
Despite a minor learning curve, this simple device enables depth control, reduces variability of manual keratectomy wound depth in rats, and decreases the risk for corneal perforation during keratectomy. Corneal defect creation was highly reproducible across different researchers and was independent of their surgical training.
This inner-stopper guarded trephine can be utilized and applied to pre-clinical testing of a wide range of corneal wound healing therapies, including but not limited to biotherapeutics, corneal prosthetics, and regenerative technologies.
在啮齿动物角膜上制造可控、可重复的角膜切除术伤口可能具有挑战性，因为它们尺寸小、厚度薄，且缺乏如真空环钻等常用于人类眼睛的工具。本研究的目的是使用一种简单、经济且定制的内塞保护环钻，在大鼠中提供一致、可重复的角膜基质缺损。
使用内塞保护环钻在大鼠角膜上制造圆形伤口。环钻术后，可用钝头刮铲手动分离去除角膜瓣。我们使用光学相干断层扫描（OCT）测量大鼠眼中诱导的缺损伤口深度。
尽管有一个较小的学习曲线，但这种简单的装置能够控制深度，减少大鼠手动角膜切除术伤口深度的变异性，并降低角膜切除术期间角膜穿孔的风险。不同研究人员制造角膜缺损的过程具有高度可重复性，且与他们的手术训练无关。
这种内塞保护环钻可用于并应用于广泛的角膜伤口愈合治疗的临床前测试，包括但不限于生物治疗、角膜假体和再生技术。
