Enzyme Activity of Cellulolytic Bacteria from Biological Education and Research Forest Floor Andalas University.

<b>Background and Objective:</b> The composition of the waste consists mostly of plant biomass. Cellulose is the largest component of plant biomass and cellulolytic bacteria are needed to degrade it. This study aimed to determine enzyme activity possessed by bacterial isolates from Biological Education and Research Forest floor Andalas University. <b>Materials and Methods:</b> The isolation stage was carried out with NA (Nutrient agar) medium, Screening with CMC (Carboxymethyl Cellulose) medium with congo red dye and enzyme activity testing was carried out using the Nelson-Somogyi method. <b>Results:</b> We found 16 bacterial isolates obtained from Biological Education and Research Forest Floor Andalas University, 10 of them were positive for cellulolytic bacteria with the highest cellulolytic index value of 2.59 on FFB 2 isolates. <b>Conclusion:</b> The bacterial isolate with the best enzyme activity value was FFB 2 isolate 0.166 U mL¹ for 72 hrs.