利用单克隆抗体对天然产物的研究：综述

Studies on Natural Products Using Monoclonal Antibodies: A Review.

作者信息

Shoyama Yukihiro

机构信息

Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan.

出版信息

Antibodies (Basel). 2021 Nov 1;10(4):43. doi: 10.3390/antib10040043.

DOI:10.3390/antib10040043

PMID:34842627

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8628510/

Abstract

An immunoblotting system ("eastern blotting") was developed for small-molecule herbal medicines like glycosides, with no conjugation function to the membrane. Briefly, the crude extracts of herb medicines were developed by thin-layer chromatography (TLC). The small-molecule herbal medicines on TLC plates were transferred to polyvinylidene fluoride (PVDF) or polyethersulfone (PES) membranes by heating. Antigen components were divided into two categories based on their function, i.e., their membrane recognizing (aglycone part) and fixing (sugar moiety) abilities. This procedure allows for the staining of only target glycosides. Double eastern blotting was developed as a further staining system for two herb medicines using a set of MAbs and substrates.

摘要

一种免疫印迹系统（“东方印迹法”）被开发用于糖苷类等无膜结合功能的小分子草药。简而言之，草药粗提物通过薄层色谱法（TLC）展开。TLC板上的小分子草药通过加热转移到聚偏二氟乙烯（PVDF）或聚醚砜（PES）膜上。抗原成分根据其功能分为两类，即它们的膜识别（苷元部分）和固定（糖部分）能力。该程序仅允许对目标糖苷进行染色。双东方印迹法作为使用一组单克隆抗体和底物对两种草药的进一步染色系统被开发出来。