Ecotoxicology Research Group, School of Science, RMIT University, Melbourne, Australia.
Ecotoxicology Research Group, School of Science, RMIT University, Melbourne, Australia.
Sci Total Environ. 2022 Feb 1;806(Pt 1):150567. doi: 10.1016/j.scitotenv.2021.150567. Epub 2021 Sep 25.
An improved microcontroller-based device for real-time biomonitoring of mussel behaviour is introduced in this study. Open source Arduino platforms were used as processing cores and infra-red (IR) sensors (with transistor output) and Hall sensors to record the cardiac activities and valve movements of mussels. Compared to the devices described in previous studies, this device has low cost, high throughput, and high portability, and can be applied to conduct real-time preliminary automatic data processing. Mediterranean mussels were exposed to Cu and microplastics and their cardiac activities and valve movements were recorded. The results demonstrated that Cu exposure caused valve closure and a drop in the heart rate, similar to the behaviour during natural periods of bradycardia in mussels. The microplastic exposures tended to cause high oscillations (low concentration of microplastics) and slow decreases (high concentration of microplastics) of the maximum valve open positions. Such oscillations and decreases appeared to reset and restart after the bradycardia period. The device has potential to measure and establish behavioural responses of mussels and other bivalves, to the stress of exposure from environmental contaminants.
本研究介绍了一种改进的基于单片机的贻贝行为实时生物监测设备。该设备使用开源的 Arduino 平台作为处理核心，并采用红外（IR）传感器（带晶体管输出）和霍尔传感器来记录贻贝的心脏活动和瓣运动。与以前研究中描述的设备相比，该设备具有低成本、高吞吐量和高便携性，可用于进行实时初步自动数据处理。本研究将地中海贻贝暴露于铜和微塑料中，并记录它们的心脏活动和瓣运动。结果表明，铜暴露导致瓣关闭和心率下降，类似于贻贝自然心动过缓期间的行为。微塑料暴露往往会导致最大瓣开口位置的高振荡（微塑料浓度低）和缓慢下降（微塑料浓度高）。这种振荡和下降似乎在心动过缓期后重置并重新开始。该设备有望测量和建立贻贝和其他双壳类动物对环境污染物暴露应激的行为反应。
