León Xavier, García Jacinto, Farré Nuria, Majercakova Katarina, Avilés-Jurado Francesc-Xavier, Quer Miquel, Camacho Mercedes
Servicio de Otorrinolaringología, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Madrid, Spain.
Servicio de Otorrinolaringología, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain.
Acta Otorrinolaringol Esp (Engl Ed). 2021 Nov-Dec;72(6):337-343. doi: 10.1016/j.otoeng.2020.05.006.
To analyse the relationship between the transcriptional expression of interleukin-8 (IL-8) and response to treatment with radiotherapy or chemo-radiotherapy in patients with squamous cell carcinoma of the head and neck (SCCHN).
Retrospective study from tumour biopsies obtained before a treatment with radiotherapy or chemo-radiotherapy in 87 patients with SCCHN. We had a sample of healthy mucosa in 35 cases. We determined the transcriptional expression of IL-8 with RT-PCR. The transcriptional expression of IL-8 was categorized according to the local control of the disease with a recursive partitioning analysis.
The transcriptional expression of IL-8 in tumour tissue was about 50 times higher than that in the samples of healthy mucosa. Patients with a high transcriptional expression of IL-8 (n = 56) had a 5-year local recurrence-free survival of 65.6%, and for patients with low expression (n = 31) it was 90.2% (P = 0.017). According to the results of a multivariate analysis, patients with high expression of IL-8 had a 4.1 higher risk of local recurrence of the tumour.
SCCHN have a significant increase in transcriptional expression of IL-8 in relation to non-tumour tissue. Tumours with high IL-8 expression have an increased risk of local recurrence after treatment with radiotherapy or chemo-radiotherapy.
分析头颈部鳞状细胞癌（SCCHN）患者白细胞介素-8（IL-8）的转录表达与放疗或放化疗治疗反应之间的关系。
对87例接受放疗或放化疗治疗前获取肿瘤活检组织的SCCHN患者进行回顾性研究。35例患者有健康黏膜样本。采用逆转录聚合酶链反应（RT-PCR）测定IL-8的转录表达。通过递归分割分析，根据疾病的局部控制情况对IL-8的转录表达进行分类。
肿瘤组织中IL-8的转录表达比健康黏膜样本中的高约50倍。IL-8转录表达高的患者（n = 56）5年局部无复发生存率为65.6%，而表达低的患者（n = 31）为90.2%（P = 0.017）。根据多因素分析结果，IL-8高表达患者肿瘤局部复发风险高4.1倍。
与非肿瘤组织相比，SCCHN中IL-8的转录表达显著增加。IL-8高表达的肿瘤在放疗或放化疗治疗后局部复发风险增加。
