Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Universidade Federal Do Rio Grande Do Sul, Rua Sarmento Leite 500, Porto Alegre, RS, 90050-170, Brazil.
Dorf Ketal Research and Development Center, Rua da Pedreira 559, Nova Santa Rita, RS, 92480-000, Brazil.
Sci Rep. 2021 Nov 29;11(1):23027. doi: 10.1038/s41598-021-02494-7.
Extreme conditions and the availability of determinate substrates in oil fields promote the growth of a specific microbiome. Sulfate-reducing bacteria (SRB) and acid-producing bacteria (APB) are usually found in these places and can harm important processes due to increases in corrosion rates, biofouling and reservoir biosouring. Biocides such as glutaraldehyde, dibromo-nitrilopropionamide (DBNPA), tetrakis (hydroxymethyl) phosphonium sulfate (THPS) and alkyl dimethyl benzyl ammonium chloride (ADBAC) are commonly used in oil fields to mitigate uncontrolled microbial growth. The aim of this work was to evaluate the differences among microbiome compositions and their resistance to standard biocides in four different Brazilian produced water samples, two from a Southeast Brazil offshore oil field and two from different Northeast Brazil onshore oil fields. Microbiome evaluations were carried out through 16S rRNA amplicon sequencing. To evaluate the biocidal resistance, the Minimum Inhibitory Concentration (MIC) of the standard biocides were analyzed using enriched consortia of SRB and APB from the produced water samples. The data showed important differences in terms of taxonomy but similar functional characterization, indicating the high diversity of the microbiomes. The APB and SRB consortia demonstrated varying resistance levels against the biocides. These results will help to customize biocidal treatments in oil fields.
极端条件和油田中确定底物的可用性促进了特定微生物组的生长。硫酸盐还原菌 (SRB) 和产酸菌 (APB) 通常存在于这些地方，由于腐蚀速率增加、生物污垢和储层生物酸化，它们可能会对重要过程造成危害。戊二醛、二溴-亚硝基丙酰胺 (DBNPA)、四羟甲基鏻硫酸盐 (THPS) 和烷基二甲基苄基氯化铵 (ADBAC) 等杀菌剂常用于油田，以减轻不受控制的微生物生长。本工作旨在评估四种不同巴西产出水中微生物组组成及其对标准杀菌剂的抗性差异，其中两种来自巴西东南部海上油田，两种来自巴西东北部不同的陆上油田。通过 16S rRNA 扩增子测序进行微生物组评估。为了评估杀菌抗性，使用来自产出水样品的 SRB 和 APB 富集共生体分析了标准杀菌剂的最小抑菌浓度 (MIC)。数据显示在分类学方面存在重要差异，但功能特征相似，表明微生物组的多样性很高。APB 和 SRB 共生体对杀菌剂表现出不同的抗性水平。这些结果将有助于定制油田中的杀菌处理。
