猪诱导多能干细胞的生成：不同方案的测试及其在成功应用中的结果。

Porcine iPSC Generation: Testing Different Protocols to a Successful Application.

机构信息

MyoCare Lab, ICREC Research Program, Germans Trias i Pujol Health Research Institute (IGTP), Badalona, Barcelona, Spain.

CIBER Cardiovascular, Instituto de Salud Carlos III, Madrid, Spain.

出版信息

Methods Mol Biol. 2022;2454:61-81. doi: 10.1007/7651_2021_446.

DOI:10.1007/7651_2021_446

PMID:34845658

Abstract

Stem cell therapy has an unparalleled potential to treat blood cancers, cardiovascular diseases and neurodegenerative conditions, among others. However, stem cell therapeutics must overcome multiple requirements before reaching clinical trials, including large animal safety and efficacy studies. In cardiovascular diseases swine models are the most widely adopted due to its great translational potential to humans. In this chapter, we will describe several protocols to induce iPSC dedifferentiation in swine fibroblasts, as well as conditioning treatments that may help in the reprogramming process.

摘要

干细胞疗法具有治疗血液癌症、心血管疾病和神经退行性疾病等多种疾病的无与伦比的潜力。然而，干细胞疗法在进入临床试验之前，必须克服多个要求，包括大型动物安全性和疗效研究。在心血管疾病中，猪模型是最广泛采用的模型，因为它具有向人类转化的巨大潜力。在本章中，我们将描述几种诱导猪成纤维细胞 iPSC 去分化的方案，以及可能有助于重编程过程的条件处理方法。