PAGE 凝胶电泳检测类病毒。

Detection of Viroids by sPAGE Gel Electrophoresis.

机构信息

Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain.

出版信息

Methods Mol Biol. 2022;2316:71-77. doi: 10.1007/978-1-0716-1464-8_7.

Abstract

Nucleic acid polyacrylamide gel electrophoresis (PAGE) has played a critical role in the identification and characterization of viroid RNAs. In addition, double PAGE has been a very efficient tool for the detection of viroids as it is sequence independent and is based on the viroid structure of covalently closed/circular molecules. sPAGE has been widely used for the identification of new viroids as well as a routine detection tool.

摘要

核酸聚丙烯酰胺凝胶电泳（PAGE）在鉴定和描述类病毒 RNA 方面发挥了关键作用。此外，双相 PAGE 是一种非常有效的检测类病毒的工具，因为它不依赖于序列，而是基于共价封闭/环形分子的类病毒结构。sPAGE 已被广泛用于鉴定新的类病毒以及常规检测工具。