Hartmann D, Adams J S, Meeker A K, Schneider M A, Lenz B F, Talmadge J E
Cancer Res. 1986 Mar;46(3):1331-8.
In this paper, we describe a study of the therapeutic parameters (dose and schedule) and immunomodulatory activity (macrophage, natural killer cell, and T-cell number and function) of polyinosinic-polycytidylic acid admixed with poly-L-lysine and solubilized with carboxymethyl cellulose [poly(I,C)-LC] in the treatment of MBL-2 tumor ascites. Tumor-bearing mice received an optimal therapeutic protocol [100 micrograms poly(I,C)-LC administered twice a wk], a maximum tolerated dose [50 micrograms poly(I,C)-LC administered daily], or the optimal immunomodulatory protocol for normal mice [10 micrograms poly(I,C)-LC administered daily]. The percentage of tumor-associated macrophages and their cytotoxic activity correlated with host survival. In addition, splenic T-cell activity correlated with host survival, and splenic natural killer cell function had a near significant correlation with host survival. These results indicate that the optimal dose and schedule of poly(I,C)-LC for immunomodulation in tumor-bearing animals are also the optimal therapeutic protocol but have less toxicity than the maximum tolerated dose.
在本文中，我们描述了一项关于聚肌苷酸-聚胞苷酸与聚-L-赖氨酸混合并用羧甲基纤维素增溶[聚（I，C）-LC]治疗MBL-2肿瘤腹水的治疗参数（剂量和给药方案）及免疫调节活性（巨噬细胞、自然杀伤细胞以及T细胞数量和功能）的研究。荷瘤小鼠接受了最佳治疗方案[每周两次给予100微克聚（I，C）-LC]、最大耐受剂量[每日给予50微克聚（I，C）-LC]或正常小鼠的最佳免疫调节方案[每日给予10微克聚（I，C）-LC]。肿瘤相关巨噬细胞的百分比及其细胞毒性活性与宿主存活率相关。此外，脾脏T细胞活性与宿主存活率相关，脾脏自然杀伤细胞功能与宿主存活率有近乎显著的相关性。这些结果表明，聚（I，C）-LC在荷瘤动物中进行免疫调节的最佳剂量和给药方案也是最佳治疗方案，但毒性低于最大耐受剂量。
