Seddik M, Seemayer T A, Lapp W S
Transplantation. 1986 Feb;41(2):238-42.
Studies were performed to determine whether pre-T cells develop normally in the bone marrow of mice displaying thymic dysplasia and T cell immunodeficiency as a consequence of a graft-versus-host (GVH) reaction. GVH reactions were induced in CBAxAF1 mice by the injection of A strain lymphoid cells. To test for the presence of pre-T cells in GVH-reactive mice, bone marrow from GVH-reactive mice (GVHBM) was injected into irradiated syngeneic F1 mice and 30-40 days later thymic morphology and function were studied. Morphology studies showed nearly normal thymic architectural restoration; moreover, such glands contained normal numbers of Thy-1-positive cells. Functional pre-T cells were evaluated by transferring thymocytes from the irradiated GVHBM-reconstituted mice into T-cell-deprived mice. These thymocytes reconstituted allograft reactivity, T helper cell function and Con A and PHA mitogen responses of T-cell-deprived mice. These results suggest that the pre-T cell population in the bone marrow is not affected by the GVH reaction. Therefore, the T cell immunodeficiency associated with the GVH reaction is not due to a deficiency of pre-T cells in the bone marrow but is more likely associated with GVH-induced thymic dysplasia.
开展了多项研究，以确定在因移植物抗宿主（GVH）反应而出现胸腺发育异常和T细胞免疫缺陷的小鼠骨髓中，前T细胞是否能正常发育。通过注射A品系淋巴细胞，在CBA×AF1小鼠中诱导GVH反应。为检测GVH反应小鼠中前T细胞的存在情况，将GVH反应小鼠的骨髓（GVHBM）注射到经辐照的同基因F1小鼠体内，30 - 40天后研究胸腺的形态和功能。形态学研究显示胸腺结构几乎恢复正常；此外，此类腺体中Thy-1阳性细胞数量正常。通过将经辐照的GVHBM重建小鼠的胸腺细胞转移到T细胞缺陷小鼠中，评估功能性前T细胞。这些胸腺细胞重建了T细胞缺陷小鼠的同种异体移植反应性、T辅助细胞功能以及对刀豆蛋白A和植物血凝素丝裂原的反应。这些结果表明，骨髓中的前T细胞群体不受GVH反应的影响。因此，与GVH反应相关的T细胞免疫缺陷并非由于骨髓中前T细胞缺乏，而更可能与GVH诱导的胸腺发育异常有关。
