Pyridine substituted BODIPYs: synthesis, characterization and cholinesterease, α-glucosidase inhibitory, DNA hydrolytic cleavage effects.

In this study, the synthesis of new monostyryl () and distyryl BODIPY dyes () containing pyridine groups has been reported for the first time. The acetylcholinesterase from (AChE), butyrylcholinesterase from equine serum (BuChE), α-glucosidase from and DNA hydrolytic cleavage actions of , , were investigated using various techniques. The results indicated that the compounds had varying inhibition properties against AChE, BuChE, and α-glucosidase. was the most potent compound on AChE with IC of 54.78 ± 4.51 µM, and Lineweaver-Burk plots indicated that the compound is bound to a site other than the active site as a noncompetitive inhibitor. The compound-protein binding experiment showed that changed the microenvironment around AChE. On the other hand, the compounds showed lower α-glucosidase inhibition than the positive control. The DNA hydrolytic cleavage effects were not observed on supercoiled plasmid DNA in the presence of the compounds as compared to negative controls. These findings suggested that might be a promising compound to treat Alzheimer's diseases.