Nicholls D G, Bernson V S, Heaton G M
Experientia Suppl. 1978;32:89-93. doi: 10.1007/978-3-0348-5559-4_9.
The proton conductance of the inner membrane of hamster brown adipose tissue mitochondria can be regulated in vitro by exogenous purine nucleotides, which bind to a component on the outer face of the inner membrane. This unique mechanism has been proposed to represent the molecular site of non-shivering thermogenesis in this tissue. Using a photo-affinity analogue of ATP, we have identified the nucleotide binding component as a protein of 32,000 daltons.
仓鼠棕色脂肪组织线粒体内膜的质子传导率在体外可被外源性嘌呤核苷酸调节，这些嘌呤核苷酸与内膜外表面的一个成分结合。有人提出，这一独特机制代表了该组织非颤抖性产热的分子位点。我们利用ATP的光亲和类似物，已将核苷酸结合成分鉴定为一种32000道尔顿的蛋白质。
