Genomic data of an environmental isolate shows high resemblance to K-12 reference strain MG1655.

species exhibits a high genomic diversification from evolution, mobile genetic elements and recombination. An environmental isolate, 'JHI_5025' from a crop trial appeared to be clonally related to the historical reference isolate K-12 strain 'MG1655', warranting further genomic analysis. Their genomes share an average nucleotide identity of 99.74% and whole genome alignment showed little rearrangement of the JHI_5025 sequence compared to the reference. Five genomic islands not in the reference aligned to other sequences in the . Isolate JHI_5025 contained K-12 F plasmid sequence and at least one complete prophage sequence. The genome and comparison dataset provides utility of JHI_5025 as a representative contemporary genetic mimic of a well-known and much used workhorse strain.