Li Yinping, Mbata George N, Shapiro-Ilan David I
Agricultural Research Station, Fort Valley State University, 1005 State University Drive, Fort Valley, GA 31030.
USDA, Agricultural Research Service, Southeastern Fruit and Tree Nut Research Laboratory, 21 Dunbar Road, Byron, GA 31008.
J Nematol. 2021 Nov 26;53. doi: 10.21307/jofnem-2021-096. eCollection 2021.
The sweetpotato whitefly, Middle East-Asia Minor 1 (MEAM1), is a major insect pest on vegetable crops worldwide. Enormous economic losses result from direct and indirect plant damage caused by MEAM1. Biological control using entomopathogenic nematodes (EPN) may be an effective alternative strategy against MEAM1 because this pest has developed resistance to most insecticides. First, nine EPN species (, , , , , , , , and ) were investigated for virulence to MEAM1 third instar nymphs on snap bean leaves under laboratory conditions. The mortality of MEAM1 nymphs was evaluated at 3 days post-inoculation (dpi). Compared to the water control, the application of the nine EPN species except resulted in significantly higher mortality of MEAM1 nymphs, such as (66.31%), (56.38%), (54.54%), and (57.80%). Subsequently, the four virulent EPN species, , , , and were evaluated further for virulence to MEAM1 nymphs on snap bean and tomato leaves. The mortality of MEAM1 nymphs was assessed at 3 dpi and 7 dpi. There were no significant differences in MEAM1 nymphal mortality between tomato and snap bean at either 3 dpi or 7 dpi. The mortality of MEAM1 nymphs caused by the application of (99.25%) was significantly higher than the other three EPN species and the water control at 7dpi. The results indicate that is a very promising biocontrol agent for management.
烟粉虱中东 - 小亚细亚1型（MEAM1）是全球蔬菜作物上的一种主要害虫。MEAM1对植物造成的直接和间接损害导致了巨大的经济损失。由于这种害虫已对大多数杀虫剂产生抗性，使用昆虫病原线虫（EPN）进行生物防治可能是对抗MEAM1的一种有效替代策略。首先，在实验室条件下，研究了9种EPN物种（,,,,,,,, 和 ）对菜豆叶片上MEAM1三龄若虫的毒力。在接种后3天（dpi）评估MEAM1若虫的死亡率。与水对照相比，除 外的9种EPN物种的施用导致MEAM1若虫的死亡率显著更高，例如 （66.31%）、 （56.38%）、 （54.54%）和 （57.80%）。随后，进一步评估了4种有毒力的EPN物种 、 、 和 对菜豆和番茄叶片上MEAM1若虫的毒力。在3 dpi和7 dpi时评估MEAM1若虫的死亡率。在3 dpi或7 dpi时，番茄和菜豆上MEAM1若虫的死亡率没有显著差异。在7 dpi时，施用 导致的MEAM1若虫死亡率（99.25%）显著高于其他三种EPN物种和水对照。结果表明， 是用于管理MEAM1的一种非常有前景的生物防治剂。
