Tata Chemicals Limited-Innovation Centre, Survey No 315, Hissa No 1-14, Ambedveth (V), Paud road, Mulshi, Pune 412111, Maharashtra, India.
ProDigest BVBA, Technologiepark 3, 9052 Zwijnaarde, Gent, Belgium.
FEMS Microbiol Lett. 2021 Dec 17;368(21-24). doi: 10.1093/femsle/fnab147.
A short-chain fructo-oligosaccharide (sc-FOS) was tested in a simulator of the human gut microbial ecosystem (SHIME) in vitro model to quantify its prebiotic effects according to Prebiotic Index (PI) and Measure of prebiotic effect (MPE) equations. FossenceTM, (sc-FOS, 0.5%) was fermented in a simulated human proximal colonic condition, using a fecal inoculum from a healthy individual. We analysed the pH reduction, substrate utilization, lactate and short-chain fatty acid (SCFA) production and microbial community modulation. Microbial fermentation of sc-FOS strongly reduced the media pH indicating the production of lactate and SCFA with accumulation of lactate and enhanced levels of acetate (34.38 ± 0.38 mM), propionate (20.93 ± 0.56 mM) and butyrate (4.93 ± 0.03 mM) compared to 18.46 ± 0.20 mM, 6.24 ± 0.10 mM and 3.3 ± 0.06 mM in the blank, respectively. Total SCFA production in test media was 61.91 ± 0.87 mM compared to 33.65 ± 0.36 mM in blank and the contribution of free-sugars present in sc-FOS to SCFAs was negligible. Modulation of the microbial community was analysed through 16S rRNA sequencing and we found that sc-FOS greatly stimulated the beneficial bacteria such as Bifidobacteria and Lactobacillus. We report the PI and MPE values for FossenceTM, as 14.9 and 0.01 respectively at the end of 24 h, which is an indicator of a strong prebiotic effect.
短链果糖低聚糖（sc-FOS）在体外模拟人类肠道微生物生态系统（SHIME）模型中进行了测试，根据益生元指数（PI）和益生元效应测量（MPE）方程来定量其益生元作用。FossenceTM（sc-FOS，0.5%）在模拟人体近端结肠条件下发酵，使用来自健康个体的粪便接种物。我们分析了 pH 值降低、底物利用、乳酸和短链脂肪酸（SCFA）产生以及微生物群落调节。sc-FOS 的微生物发酵强烈降低了培养基的 pH 值，表明产生了乳酸和 SCFA，乳酸积累，并且乙酸（34.38±0.38 mM）、丙酸（20.93±0.56 mM）和丁酸（4.93±0.03 mM）的水平增强，与空白相比，分别为 18.46±0.20 mM、6.24±0.10 mM 和 3.3±0.06 mM。测试培养基中的总 SCFA 产量为 61.91±0.87 mM，而空白中的产量为 33.65±0.36 mM，sc-FOS 中存在的游离糖对 SCFAs 的贡献可以忽略不计。通过 16S rRNA 测序分析微生物群落的调节，我们发现 sc-FOS 极大地刺激了双歧杆菌和乳杆菌等有益细菌。我们报告 FossenceTM 的 PI 和 MPE 值分别为 14.9 和 0.01，在 24 小时结束时，这是强烈益生元作用的指标。
