Husted S, Hjort T
Int J Fertil. 1975;20(2):97-105.
The relation between sperm antibodies (agglutinins and IF-antibodies) in serum and seminal plasma was studied in three selected groups of male partners in infertile couples with respect to specificity, concentrations and immunoglobulin classes. Agglutinins were found in seminal plasma only when they were also present in serum, but there was no strict correlation between the titres in seminal plasma and serum although serum titres were always the higher ones. However, in cases with tail-to-tail agglutinins a difference of more than 2 fourfold steps between the titres was never found; spontaneous agglutination in the ejaculate generally occurred when the seminal plasma titre was 64 or more, and in 6 patients with high seminal plasma titres IgA was detected in the midpiece region of ejaculated spermatozoa. In contrast, head-to-head agglutinins were found in seminal plasma in only one of 3 patients with these agglutinins in serum, and only in a low titre. Absorption of IgG of IgG with protein-A-producing Staphylococcus aureus showed that tail-to-tail agglutinins in serum were IgG antibodies, whereas they could be characterized as "non-IgA-in seminal plasma, suggesting a local production of these antibodies. IF-antibodies were rarely found in seminal plasma and seemed to be of little significance in relation to infertility in men.
针对不育夫妇中三组选定男性伴侣的血清和精浆中的精子抗体（凝集素和免疫荧光抗体），研究了其特异性、浓度和免疫球蛋白类别之间的关系。仅当血清中存在凝集素时，精浆中才会发现凝集素，但精浆和血清中的滴度之间没有严格的相关性，尽管血清滴度总是更高。然而，在出现尾对尾凝集素的情况下，滴度之间从未发现超过2个四倍级差；当精浆滴度为64或更高时，射精中通常会出现自发凝集，并且在6名精浆滴度高的患者中，在射出精子的中段区域检测到了IgA。相比之下，在血清中有头对头凝集素的3名患者中，只有1名患者的精浆中发现了头对头凝集素，且滴度较低。用产生蛋白A的金黄色葡萄球菌吸收IgG表明，血清中的尾对尾凝集素是IgG抗体，而在精浆中它们可被表征为“非IgA”，这表明这些抗体是局部产生的。免疫荧光抗体在精浆中很少发现，似乎与男性不育关系不大。
