Ge Huifang, Zhang Biying, Li Ting, Yang Qi, Tang Yuanhu, Liu Jingbo, Zhang Ting
Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun, 130062, People's Republic of China.
Food Funct. 2021 Dec 13;12(24):12774-12787. doi: 10.1039/d1fo03095g.
Active peptides, as an alternative nutrition supplement, have been confirmed to have beneficial efficacy against acute colitis. Herein, egg white peptides (EWPs) were used as a nutritional supplement to relieve dextran sulfate sodium-induced acute colitis symptoms. The potential multi-component synergetic pharmacological intervention mechanism of EWPs was investigated on the basis of pharmacology, bioinformatics analysis, and molecular docking. experiments demonstrated that the migration rate of HSF cells was enhanced 5.30-fold upon treatment with EWPs relative to the control group. After administration with EWPs, colitis symptoms were alleviated in a dose-dependent manner and the serum amino acid content was significantly enhanced, especially for Ala, Leu, Ser, Thr, and Met. Four peptides identified from EWPs showed a total of 52 acute colitis-related potential targets (Fit score >3.8) with network pharmacology analysis, and the targets participated in 31 signaling pathways ( < 0.001). Among these pathways, PI3K-Akt, VEGF, Ras, TNF, and MAPK signaling pathways may exert essential anti-inflammatory effects and accelerate repairing intestinal mucosa. Molecular docking showed that the majority binding energy of peptides-targets was between -10.35 kcal mol and -18.72 kcal mol, and peptides mainly interacted with the core targets (Btk, Gstm1, and Rac1) by hydrogen-bonding interactions. The current study confirmed that EWPs as supplementary nutrition can alleviate acute colitis.
活性肽作为一种替代性营养补充剂，已被证实对急性结肠炎具有有益功效。在此，卵清蛋白肽（EWP）被用作营养补充剂以缓解葡聚糖硫酸钠诱导的急性结肠炎症状。基于药理学、生物信息学分析和分子对接，研究了EWP潜在的多成分协同药理干预机制。实验表明，与对照组相比，用EWP处理后HSF细胞的迁移率提高了5.30倍。给予EWP后，结肠炎症状呈剂量依赖性缓解，血清氨基酸含量显著增加，尤其是丙氨酸、亮氨酸、丝氨酸、苏氨酸和蛋氨酸。通过网络药理学分析，从EWP中鉴定出的四种肽共有52个与急性结肠炎相关的潜在靶点（拟合分数>3.8），这些靶点参与了31条信号通路（<0.001）。在这些通路中，PI3K-Akt、VEGF、Ras、TNF和MAPK信号通路可能发挥重要的抗炎作用并加速肠黏膜修复。分子对接表明，肽-靶点的大多数结合能在-10.35 kcal/mol和-18.72 kcal/mol之间，肽主要通过氢键相互作用与核心靶点（Btk、Gstm1和Rac1）相互作用。当前研究证实，EWP作为补充营养可缓解急性结肠炎。
