Rinchik E M, Amos D B
J Immunol. 1986 Mar 15;136(6):1992-8.
During the course of screening new T-H-2 region congenic strains of mice constructed from the C57BL/6 and B6-H-2k strains, a new cell surface polymorphism, designated dtc-1, was identified by cell-mediated lympholysis techniques. The dtc-1 antigen can be found on both Con A- and LPS-stimulated lymphoblasts, peritoneal macrophages, and SV40-transformed mouse embryo fibroblasts. Lysis of dtc-1+ targets by CTL is H-2Dk restricted. All inbred strains tested are dtc-1+, with the exception of the B6-H-2k strain, which is dtc-1-, and several congenic strains directly derived from B6-H-2k. Because B6/Boy and AKR/Boy, the parents of the B6-H-2k strain, are dtc-1+, the dtc-1- phenotype may be the result of mutation in the locus specifying the cell surface molecule that carries this antigen. Segregation analysis of the dtc-1+/dtc-1- polymorphism demonstrated that this locus is not linked to T or H-2. The dtc-1 antigen thus identifies yet another cell surface polymorphism and adds another immunologically defined genetic marker to the murine genome. Furthermore, the dtc-1 system indicates the need for reevaluation and restandardization of congenic strains of mice derived from the B6-H-2k congenic strain.
在筛选由C57BL/6和B6-H-2k品系构建的新的T-H-2区域同源近交系小鼠的过程中，通过细胞介导的淋巴细胞溶解技术鉴定出一种新的细胞表面多态性，命名为dtc-1。dtc-1抗原可在刀豆蛋白A和脂多糖刺激的淋巴母细胞、腹腔巨噬细胞以及SV40转化的小鼠胚胎成纤维细胞上发现。细胞毒性T淋巴细胞（CTL）对dtc-1+靶细胞的溶解受H-2Dk限制。除了B6-H-2k品系及其直接衍生的几个同源近交系为dtc-1-外，所有测试的近交系均为dtc-1+。由于B6-H-2k品系的亲本B6/Boy和AKR/Boy是dtc-1+，因此dtc-1-表型可能是指定携带该抗原的细胞表面分子的基因座发生突变的结果。dtc-1+/dtc-1-多态性的分离分析表明，该基因座与T或H-2不连锁。因此，dtc-1抗原鉴定出另一种细胞表面多态性，并为小鼠基因组增添了另一个免疫定义的遗传标记。此外，dtc-1系统表明需要对源自B6-H-2k同源近交系的小鼠同源近交系进行重新评估和重新标准化。
