从凝集素刺激的正常人淋巴细胞培养基中简单制备两步法纯化白细胞介素-2。

Simple preparative two-step purification of interleukin-2 from culture medium of lectin stimulated normal human lymphocytes.

作者信息

Körner I J, Dettmer R, Kopp J, Gaestel M, Malz W

出版信息

J Immunol Methods. 1986 Mar 13;87(2):185-91. doi: 10.1016/0022-1759(86)90529-6.

Abstract

A procedure has been developed for the rapid purification of human IL-2 using reversed-phase liquid chromatography on LiChroprep RP-8 and Separon SGX C-18. The resulting IL-2 has a specific activity of 1 X 10(7) U/mg protein and reveals in SDS-PAGE 2 molecular weight forms (approximately 14 kDa and approximately 16 kDa) under reducing and non-reducing conditions. Both forms are biologically active. In 2-dimensional electrophoresis purified IL-2 shows a complex pattern of protein spots located in the IL-2 region which may be due to differences in glycosylation.

摘要

已开发出一种程序，可使用LiChroprep RP - 8和Separon SGX C - 18进行反相液相色谱法快速纯化人白细胞介素-2（IL - 2）。所得的IL - 2比活性为1×10⁷U/mg蛋白质，在还原和非还原条件下的SDS - PAGE中显示出2种分子量形式（约14 kDa和约16 kDa）。两种形式均具有生物活性。在二维电泳中，纯化的IL - 2在IL - 2区域显示出复杂的蛋白质斑点模式，这可能是由于糖基化差异所致。