Kermani-Arab V, Uittenbogaart C, Bramhall J
Thymus. 1986;8(1-2):3-11.
We have developed a simple protocol for the routine purification of essentially homogeneous human interleukin 2. The procedures applied include ammonium sulfate precipitation, ACA-54 gel filtration, ultrafiltration and chromatofocusing. The product has a molecular weight of 14 000, as determined by electrophoretic mobility, and is free of interleukin 1, interferon, granulocyte and monocyte stimulating factors, B cell growth factor and phytohemagglutinin. The method is efficient, rapid and reproduceable and provides a helpful method for preparation of IL-2 for biochemical and biological studies at moderate cost and without the use of complex equipment.
我们已经开发出一种简单的方案，用于常规纯化基本均一的人白细胞介素2。所采用的步骤包括硫酸铵沉淀、ACA - 54凝胶过滤、超滤和色谱聚焦。通过电泳迁移率测定，该产物的分子量为14000，且不含白细胞介素1、干扰素、粒细胞和单核细胞刺激因子、B细胞生长因子及植物血凝素。该方法高效、快速且可重复，以适中的成本且无需使用复杂设备，为生化和生物学研究制备白细胞介素2提供了一种有用的方法。
