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基于频域空域联合优化的非线性结构光照明显微镜超分辨率图像改善方法

Frequency-spatial domain joint optimization for improving super-resolution images of nonlinear structured illumination microscopy.

出版信息

Opt Lett. 2021 Dec 1;46(23):5842-5845. doi: 10.1364/OL.441160.

Abstract

Introducing nonlinear fluorophore excitation into structured illumination microscopy (SIM) can further extend its spatial resolution without theoretical limitation. However, it is a great challenge to recover the weak higher-order harmonic signal and reconstruct high-fidelity super-resolution (SR) images. Here, we proposed a joint optimization strategy in both the frequency and spatial domains to reconstruct high-quality nonlinear SIM (NL-SIM) images. We demonstrate that our method can reconstruct SR images with fewer artifacts and higher fidelity on the BioSR dataset with patterned-activation NL-SIM. This method could robustly overcome one of the long-lived obstacles on NL-SIM imaging, thereby promoting its wide application in biology.

摘要

将非线性荧光体激发引入结构光照明显微镜(SIM)中,可以在不受到理论限制的情况下进一步扩展其空间分辨率。然而,从弱的高阶谐频信号中恢复并重建高保真超分辨率(SR)图像仍然是一个巨大的挑战。在这里,我们提出了一种在频率和空间域中进行联合优化的策略,以重建高质量的非线性 SIM(NL-SIM)图像。我们在具有图案化激活 NL-SIM 的 BioSR 数据集上证明,我们的方法可以在更少的伪影和更高的保真度下重建 SR 图像。该方法可以稳健地克服 NL-SIM 成像中的一个长期存在的障碍,从而促进其在生物学中的广泛应用。

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