Gan Chunchun, Jin Zhan, Wei Xiaopeng, Jin Meina
School of Medicine, Quzhou College of Technology, China.
School of Pharmacy, Tianjin Medical University, Tianjin, China.
Folia Histochem Cytobiol. 2021;59(4):226-235. doi: 10.5603/FHC.a2021.0023. Epub 2021 Dec 1.
Actinidia chinensis Planch. root extract (acRoots), known as a traditional Chinese medicine (TCM), has shown antitumor efficacy in various types of human cancers. However, its role and underlying mechanisms in breast cancer (BCa) have not been elucidated.
In the present study, the effects of acRoots on cell viability, apoptosis, migration and invasion were analyzed by MTT assay, colony formation, flow cytometry, wound healing and Transwell assay in MDA-MB-231 and MDA-MB-453 breast cancer cell lines. The expression levels of relevant proteins were determined by Western blot assay.
The results revealed that acRoots inhibited proliferation, migration, and invasion and promoted apoptosis of BCa cells. Moreover, acRoots decreased the expression of cyclin D1, survivin, Bcl-2, N-cadherin, and Snail and increased the expression of Bax and E-cadherin in MDA-MB-231 and MDA-MB-453 cells. AcRoots inhibited the AKT/GSK-3b pathway by decreasing the levels of phosphorylated AKT, phosphorylated GSK-3b and b-catenin.
The described effects of acRoots on the cultured BCa cells suggest that they may be mediated by the inhibition of the AKT/GSK-3b signaling pathway. Thus, further studies are warranted to test the possibility that AcRoots may be used as a promising anticancer agent for breast cancer treatment.
中华猕猴桃根提取物（acRoots）作为一种传统中药，已在多种人类癌症中显示出抗肿瘤功效。然而，其在乳腺癌（BCa）中的作用及潜在机制尚未阐明。
在本研究中，通过MTT法、集落形成实验、流式细胞术、伤口愈合实验和Transwell实验，分析acRoots对MDA-MB-231和MDA-MB-453乳腺癌细胞系的细胞活力、凋亡、迁移和侵袭的影响。通过蛋白质印迹法测定相关蛋白的表达水平。
结果显示，acRoots抑制BCa细胞的增殖、迁移和侵袭，并促进其凋亡。此外，acRoots降低了MDA-MB-231和MDA-MB-453细胞中细胞周期蛋白D1、生存素、Bcl-2、N-钙黏蛋白和Snail的表达，增加了Bax和E-钙黏蛋白的表达。acRoots通过降低磷酸化AKT、磷酸化GSK-3β和β-连环蛋白的水平来抑制AKT/GSK-3β信号通路。
acRoots对培养的BCa细胞的上述作用表明，这些作用可能是通过抑制AKT/GSK-3β信号通路介导的。因此，有必要进一步研究acRoots作为一种有前景的乳腺癌治疗抗癌药物的可能性。
