College of Life Science, Northeast Agricultural University, Harbin 150030, China.
College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Bioresour Technol. 2021 Dec;342:125985. doi: 10.1016/j.biortech.2021.125985. Epub 2021 Sep 20.
The final products of shikimic acid pathway, aromatic amino acids (AAA), can be used as humic acid (HA) precursors. Therefore, the aim of this study was to explore the contribution of shikimic acid pathway on the formation of HA during composting. Four composting treatments were carried out in this study, including the control, biochar addition, montmorillonite addition, biochar and montmorillonite combined addition. The results showed that the correlations between AAA and HA were enhanced during combined addition composting, and functional microorganisms involved in the shikimic acid pathway increased. In addition, random forest model suggested that 63.3% of the top 30 genera contributing to the HA formation were functional microorganisms involved in the shikimic acid pathway, which fully proved the critical role of shikimic acid pathway. Therefore, this study provided a new perspective for revealing the crucial factors that promoted the formation of HA during composting.
莽草酸途径的终产物芳香族氨基酸（AAA）可以作为腐殖酸（HA）的前体。因此，本研究旨在探索莽草酸途径在堆肥过程中形成 HA 的贡献。本研究共进行了四种堆肥处理，包括对照、生物炭添加、蒙脱石添加、生物炭和蒙脱石联合添加。结果表明，在联合添加堆肥过程中，AAA 与 HA 之间的相关性增强，参与莽草酸途径的功能微生物增加。此外，随机森林模型表明，对 HA 形成贡献最大的前 30 个属中有 63.3%是参与莽草酸途径的功能微生物，充分证明了莽草酸途径的关键作用。因此，本研究为揭示促进堆肥过程中 HA 形成的关键因素提供了新的视角。
