Orr N H, DiBerardino M A, McKinnell R G
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1369-73. doi: 10.1073/pnas.83.5.1369.
Seven nuclear lines derived from erythrocyte nuclei of Rana pipiens were produced by serial nuclear transplantation into oocytes and eggs. Even at the termination of the experiments, embryos and tadpoles developed in the eighth transplant generations. Thus, there was no evidence that the mitotic progeny of the erythrocyte nuclei lost their ability to replicate their genomes and continue cell cycling. We conclude that the genome of noncycling and terminally differentiated erythrocytes maintains its potential for widespread replication and extensive reversal of gene function in excess of a hundred (centuplicate) cell cycles.
