Daniel K. Inouye Center for Microbial Oceanography: Research and Education, Department of Oceanography and Sea Grant College Program, University of Hawai'i at Mānoa, Honolulu, HI, USA.
Department of Biology, California State University, Northridge, CA, USA.
NPJ Biofilms Microbiomes. 2021 Dec 1;7(1):84. doi: 10.1038/s41522-021-00252-1.
Work on marine biofilms has primarily focused on host-associated habitats for their roles in larval recruitment and disease dynamics; little is known about the factors regulating the composition of reef environmental biofilms. To contrast the roles of succession, benthic communities and nutrients in structuring marine biofilms, we surveyed bacteria communities in biofilms through a six-week succession in aquaria containing macroalgae, coral, or reef sand factorially crossed with three levels of continuous nutrient enrichment. Our findings demonstrate how biofilm successional trajectories diverge from temporal dynamics of the bacterioplankton and how biofilms are structured by the surrounding benthic organisms and nutrient enrichment. We identify a suite of biofilm-associated bacteria linked with the orthogonal influences of corals, algae and nutrients and distinct from the overlying water. Our results provide a comprehensive characterization of marine biofilm successional dynamics and contextualize the impact of widespread changes in reef community composition and nutrient pollution on biofilm community structure.
海洋生物膜的研究主要集中在宿主相关生境上，因为它们在幼虫补充和疾病动态方面发挥着作用；而对于调节珊瑚礁环境生物膜组成的因素却知之甚少。为了对比演替、底栖群落和营养物质在海洋生物膜结构中的作用，我们通过在包含大型藻类、珊瑚或珊瑚砂的水族箱中进行为期六周的演替实验，调查了生物膜中的细菌群落，该实验采用了三水平的连续营养盐富化因子与珊瑚、藻类和底栖生物及营养盐富化因子的交叉实验设计。我们的研究结果表明了生物膜演替轨迹如何与浮游细菌的时间动态分道扬镳，以及生物膜是如何被周围的底栖生物和营养盐富化所构建的。我们确定了一套与珊瑚、藻类和营养盐的正交影响相关的生物膜相关细菌，并与上层水区分开来。我们的研究结果全面描述了海洋生物膜演替动态，并阐明了珊瑚礁群落组成和营养污染的广泛变化对生物膜群落结构的影响。
