Slack B E, Bell J E, Benos D J
Am J Physiol. 1986 Feb;250(2 Pt 1):C340-4. doi: 10.1152/ajpcell.1986.250.2.C340.
The effects of inositol-1,4,5-trisphosphate (IP3) and of diacylglycerol (DAG) and its analogues on the membrane potential of eggs from the sea urchin Strongylocentrotus purpuratus were examined. Injection of IP3 into eggs resulted in a change in membrane potential that was similar in magnitude and time course to the fertilization potential elicited by sperm attachment. In low-calcium seawater, IP3 injection elicited a partial response. DAG and its analogues phorbol myristyl acetate and 1-oleoyl-2-acetylglycerol did not affect membrane potential either when applied by perfusion or when injected. The results indicate that IP3, but not DAG or its analogues, may be involved in the generation of the fertilization potential triggered by the interaction of sperm with sea urchin eggs.
研究了肌醇-1,4,5-三磷酸（IP3）、二酰甘油（DAG）及其类似物对紫海胆卵膜电位的影响。向卵中注射IP3会导致膜电位发生变化，其幅度和时间进程类似于精子附着引发的受精电位。在低钙海水中，注射IP3引发了部分反应。通过灌注或注射应用DAG及其类似物佛波酯和1-油酰基-2-乙酰甘油时，均不影响膜电位。结果表明，IP3而非DAG或其类似物可能参与了精子与海胆卵相互作用触发的受精电位的产生。
