大鼠中毒性肝炎肝微粒体单加氧酶体系及一氧化氮代谢和倍半萜烯内酯的作用。

Monooxygenase System and NO Metabolism in Liver Microsomes of Rats with Toxic Hepatitis and the Effect of Sesquiterpene Lactones.

机构信息

Institute of Molecular Pathology and Pathomorphology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.

Institute of Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.

出版信息

Bull Exp Biol Med. 2021 Dec;172(2):133-136. doi: 10.1007/s10517-021-05349-3. Epub 2021 Dec 2.

DOI:10.1007/s10517-021-05349-3

PMID:34853965

Abstract

We analyzed changes in activities of enzymes of phases I and II of xenobiotic biotransformation and parameters of NO metabolism in liver microsomes of rats with toxic CCl-induced hepatitis after a 14-day course of sesquiterpene lactones from Artemisia leucodes (10 mg/kg). It was found that toxic hepatitis was associated with significant inhibition of NADPH-cytochrome c-reductase, benzo(a)pyrene hydroxylase, and NADPH-diaphorase, reduced cytochrome P-450 content, and enhanced induction of nitrate/nitrite reductase with accumulation of NO metabolites in the liver. Administration of sesquiterpene lactones stimulated activity of the studied components of the cytochrome P-450 system and promoted recovery of the NOergic system components; the effects were most pronounced in 7 and 14 days after treatment.

摘要

我们分析了在为期 14 天的 Artemisia leucodes 倍半萜内酯（10mg/kg）治疗后，具有 CCl 诱导的毒性肝炎的大鼠肝微粒体中外源生物转化相 I 和 II 酶的活性和 NO 代谢参数的变化。结果发现，毒性肝炎与 NADPH-细胞色素 c 还原酶、苯并（a）芘羟化酶和 NADPH-黄递酶的显著抑制、细胞色素 P-450 含量降低以及硝酸盐/亚硝酸盐还原酶的诱导增强有关，导致 NO 代谢物在肝脏中积累。倍半萜内酯的给药刺激了细胞色素 P-450 系统的研究成分的活性，并促进了 NO 能系统成分的恢复；在治疗后 7 天和 14 天最为明显。