State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Arch Virol. 2022 Feb;167(2):603-606. doi: 10.1007/s00705-021-05319-0. Epub 2021 Dec 2.
Sclerotinia sclerotiorum reovirus 1 (SsReV1) was previously reported to infect hypovirulent strain SCH941 of the phytopathogenic fungus Sclerotinia sclerotiorum and to contain 11 double-stranded RNA (dsRNA) segments (S1-S11). Here, we report that SsReV1 is actually composed of 12 dsRNA segments instead of 11. The full-length nucleotide sequence of the twelfth segment (S12) was determined using a combination of RACE and high-throughput sequencing methods. S12 is 1217 nucleotides in length and has highly conserved terminal sequences that resemble those of the other 11 segments of SsReV1. S12 contains a single open reading frame encoding a protein (VP12) of 311 amino acids. Although regular BLAST analysis did not reveal any similarity of VP12 to known sequences, it was found to be homologous to the VP11 of Colorado tick fever virus of the genus Coltivirus when a hidden-Markov-model-based HHpred analysis was performed. A single-protoplast regeneration experiment suggested that S12 and S2 were maintained or lost in parallel. In summary, the SsReV1 genome consists of 12 dsRNA segments.
茎点霉属核多角体病毒 1（SsReV1）先前被报道感染植物病原菌核盘菌的弱毒菌株 SCH941，并包含 11 个双链 RNA（dsRNA）片段（S1-S11）。在这里，我们报告称 SsReV1 实际上由 12 个 dsRNA 片段组成，而不是 11 个。使用 RACE 和高通量测序方法的组合，确定了第十二个片段（S12）的全长核苷酸序列。S12 长 1217 个核苷酸，具有高度保守的末端序列，类似于 SsReV1 的其他 11 个片段。S12 包含一个单一的开放阅读框，编码一个由 311 个氨基酸组成的蛋白（VP12）。尽管常规 BLAST 分析没有显示 VP12 与已知序列有任何相似性，但当进行基于隐马尔可夫模型的 HHpred 分析时，发现它与科罗拉多蜱热病毒属的 Coltivirus 的 VP11 同源。单个原生质体再生实验表明，S12 和 S2 是同时被维持或丢失的。总之，SsReV1 基因组由 12 个 dsRNA 片段组成。
