Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang, China.
Biol Trace Elem Res. 2022 Oct;200(10):4385-4394. doi: 10.1007/s12011-021-03032-2. Epub 2021 Dec 2.
Iodine is an essential trace element in the human body. Severe maternal iodine deficiency during pregnancy leads to obvious intellectual disability in the offspring. The effects of iodine deficiency on brain development have been demonstrated, but there is no clear evidence of the effects of iodine excess on brain development. To clarify the effects of iodine excess on the brain development of offspring and to provide clues to the mechanisms underlying the effects of iodine deficiency and iodine excess on the brain development of offspring. In this study, animal models with different iodine intakes were constructed using potassium iodate (KIO). The models included four experimental groups (low-iodine group one (LI, 0μg/L iodine), low-iodine group two (LII, 5μg/L iodine), high-iodine group one (HI, 3000μg/L iodine), and high-iodine group two (HII, 10000μg/L iodine)) and one control group (NI, 100μg/L iodine). There were 20 female rats in each group, and 8 offspring were chosen from each group following birth to assess metabolic alterations. The metabolites of subsets of brain hippocampal tissue were profiled by ultra-performance liquid chromatography-linked electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOFMS) and the results were subjected to multivariate data analysis. Differential substances were screened by t test (p<0.05), principal component analysis (PCA), and partial least squares analysis (PLS-DA, VIP>1). The thyroid function of the female rats in the experimental group was abnormally changed. Metabolic analysis showed that the five groups were separated which revealed significant differences in hippocampal tissue metabolism among the five groups of offspring. A total of 12 potential metabolites were identified, with the majority of them being related to amino acid and energy metabolism. These metabolites are involved in various metabolic pathways, are interrelated, and may play a function in brain development. Our study highlights changes in metabolites and metabolic pathways in the brain hippocampus of offspring rats with different iodine intakes compared to controls, revealing new insights into hippocampal metabolism in offspring rats and new relevant targets.
碘是人体必需的微量元素。孕妇严重碘缺乏会导致后代明显的智力障碍。碘缺乏对脑发育的影响已得到证实，但目前尚不清楚碘过量对脑发育的影响。为了阐明碘过量对后代脑发育的影响，并为碘缺乏和碘过量对后代脑发育影响的机制提供线索。在这项研究中，使用碘酸钾（KIO）构建了不同碘摄入量的动物模型。模型包括四个实验组（低碘组一（LI，0μg/L 碘）、低碘组二（LII，5μg/L 碘）、高碘组一（HI，3000μg/L 碘）和高碘组二（HII，10000μg/L 碘））和一个对照组（NI，100μg/L 碘）。每组有 20 只雌性大鼠，每组选择 8 只后代进行代谢改变评估。采用超高效液相色谱-电喷雾电离四极杆飞行时间质谱联用（UPLC-ESI-QTOFMS）对脑海马组织亚群的代谢物进行分析，并对多变量数据分析结果进行分析。通过 t 检验（p<0.05）、主成分分析（PCA）和偏最小二乘分析（PLS-DA，VIP>1）筛选差异物质。实验组雌性大鼠甲状腺功能异常改变。代谢分析显示，五组分离，五组后代海马组织代谢存在显著差异。共鉴定出 12 种潜在代谢物，其中大部分与氨基酸和能量代谢有关。这些代谢物涉及多种代谢途径，相互关联，可能在脑发育中发挥作用。本研究突出了不同碘摄入的后代大鼠海马组织代谢物和代谢途径的变化，为后代大鼠海马代谢提供了新的见解，并发现了新的相关靶点。
