School of Dentistry, Centre for Orofacial Regeneration, Reconstruction and Rehabilitation (COR3), Epigenetics Nanodiagnostics and therapeutics Group, The University of Queensland, Herston, QLD, Australia.
School of Dentistry, The University of Queensland, Herston, QLD, Australia.
J Periodontal Res. 2022 Apr;57(2):294-304. doi: 10.1111/jre.12959. Epub 2021 Dec 2.
To investigate the effect of Hfe gene mutation on the distribution of iron and periodontal bone loss in periodontal tissues.
It remains unclear how tissue iron loading affects the periodontium architectures in a genetic animal model of hereditary haemochromatosis (HH).
Male C57BL/6 Hfe (8 weeks old) and wild-type (WT) mice were utilized to examine the iron distribution in periodontal tissues, as well as periodontal tissues changes using micro-computed tomography and histomorphometric analysis. Furthermore, tissue inflammatory mediators, bone markers and periodontal pathogens were carried out in PFA-fixed paraffin-embedded tissues using ELISA, RT-qPCR and genomic DNA qPCR, respectively.
Excessive iron deposition was found in the periodontal ligament, gingiva and alveolar bone in Hfe mice relative to their WT counterparts. This, in turn, was associated with significant periodontal bone loss, increased cemento-enamel junction-alveolar bone crest distance and decreased expression of molecules involved in bone development and turnover. Furthermore, the pro-inflammatory cytokine - interleukin 6 and periodontal bacteria - Campylobacter rectus were significantly increased in Hfe mice compared with WT controls.
Our results suggest that the iron loading in a mouse model of HH decreases alveolar bone formation and leads to alterations in the inflammatory state in the periodontium. Periodontal health should be assessed during the clinical assessment of HFE-HH patients.
研究 Hfe 基因突变对牙周组织中铁分布和牙周骨丢失的影响。
遗传性血色素沉着症（HH）的遗传动物模型中，组织铁负荷如何影响牙周结构尚不清楚。
利用雄性 C57BL/6 Hfe （8 周龄）和野生型（WT）小鼠，通过 micro-CT 和组织形态计量学分析，检测牙周组织中铁的分布以及牙周组织的变化。此外，通过 ELISA、RT-qPCR 和基因组 DNA qPCR 分别在 PFA 固定的石蜡包埋组织中检测组织炎症介质、骨标志物和牙周病原体。
与 WT 相比，Hfe 小鼠的牙周韧带、牙龈和牙槽骨中发现了过多的铁沉积。这反过来又与显著的牙周骨丢失、牙釉质-牙槽骨嵴距离增加和参与骨发育和转换的分子表达减少有关。此外，与 WT 对照组相比，Hfe 小鼠中的促炎细胞因子-白细胞介素 6 和牙周细菌-直肠弯曲杆菌显著增加。
我们的研究结果表明，HH 小鼠模型中的铁负荷减少了牙槽骨形成，并导致牙周组织中炎症状态的改变。在评估 HFE-HH 患者的临床情况时，应评估牙周健康状况。
