Clinical Neurology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Physiology-Pharmacology-Medical Physic, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Brain Behav. 2022 Jan;12(1):e2447. doi: 10.1002/brb3.2447. Epub 2021 Dec 2.
In the present study, we aimed to determine whether intraperitoneal injection of platelet-rich plasma (PRP) could have a neuroprotective effect on learning, memory, and synaptic plasticity impairment as well as hippocampal apoptosis in rats with hepatic encephalopathy induced by bile duct ligated (BDL).
The rats were divided into four groups: the control, sham, BDL+ V (vehicle), and BDL+ PRP. The BDL rats were treated with PRP immediately after the surgery, and the injection was done every 3 days for 30 days. The passive avoidance and Morris water maze tests were used for the evaluation of learning and memory. The long-term potentiation (LTP), basal-synaptic transmission, and paired-pulse ratio, as an index for measurement of neurotransmitter release probability, were evaluated by field-potential recording. After taking a blood sample for assessment of the liver enzymes, the animals were sacrificed and their hippocampus was removed for evaluation of cleaved caspase-3 by Western blot.
Serological assessment of the liver function showed that BDL severely impaired the liver function. Also, PRP treatment could partially improve the liver dysfunction along with recovery in fear memory and spatial learning memory performance, LTP, basal-synaptic transmission, and neurotransmitter release probability. PRP-treated rats also showed a significant reduction in neuronal apoptosis in the CA1 area.
The results of this study suggest that PRP improves cognitive performance and synaptic plasticity in BDL rats via direct neuroprotective property and/or indirectly by improvement of hepatic dysfunction.
在本研究中，我们旨在确定富血小板血浆（PRP）腹腔内注射是否对胆管结扎（BDL）诱导的肝性脑病大鼠的学习、记忆和突触可塑性损伤以及海马细胞凋亡具有神经保护作用。
将大鼠分为四组：对照组、假手术组、BDL+V（载体）组和 BDL+PRP 组。BDL 大鼠在手术后立即接受 PRP 治疗，每 3 天注射一次，共 30 天。采用被动回避和 Morris 水迷宫测试评估学习和记忆。通过场电位记录评估长时程增强（LTP）、基础突触传递和成对脉冲比（作为测量神经递质释放概率的指标）。采集血样评估肝功能后，处死动物并取出海马，通过 Western blot 评估裂解半胱天冬酶-3。
肝功能的血清学评估表明，BDL 严重损害了肝功能。此外，PRP 治疗可部分改善肝功能，同时恢复恐惧记忆和空间学习记忆表现、LTP、基础突触传递和神经递质释放概率。PRP 治疗组大鼠 CA1 区神经元凋亡也明显减少。
本研究结果表明，PRP 通过直接的神经保护作用和/或通过改善肝功能，改善 BDL 大鼠的认知功能和突触可塑性。
