Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
Medical Scientist Training Program, Mayo Clinic, Rochester, Minnesota 55905, United States.
Anal Chem. 2021 Dec 28;93(51):16987-16994. doi: 10.1021/acs.analchem.1c02131. Epub 2021 Dec 2.
Here, we present the development of a novel voltammetric technique, N-shaped multiple cyclic square wave voltammetry (N-MCSWV) and its application . It allows quantitative measurements of tonic extracellular levels of serotonin with mitigated fouling effects. N-MCSWV enriches the electrochemical information by generating high dimensional voltammograms, which enables high sensitivity and selectivity against 5-hydroindoleacetic acid (5-HIAA), dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), histamine, ascorbic acid, norepinephrine, adenosine, and pH. Using N-MCSWV, in combination with PEDOT:Nafion-coated carbon fiber microelectrodes, a tonic serotonin concentration of 52 ± 5.8 nM ( = 20 rats, ±SEM) was determined in the substantia nigra pars reticulata of urethane-anesthetized rats. Pharmacological challenges with dopaminergic, noradrenergic, and serotonergic synaptic reuptake inhibitors supported the ability of N-MCSWV to selectively detect tonic serotonin levels . Overall, N-MCSWV is a novel voltammetric technique for analytical quantification of serotonin. It offers continuous monitoring of changes in tonic serotonin concentrations in the brain to further our understanding of the role of serotonin in normal behaviors and psychiatric disorders.
在这里，我们提出了一种新型的伏安技术，即 N 形多循环方波伏安法（N-MCSWV）及其应用。它允许定量测量 5-羟色胺的紧张型细胞外水平，同时减轻了污垢效应。N-MCSWV 通过生成高维伏安图来丰富电化学信息，从而实现了对 5-羟吲哚乙酸（5-HIAA）、多巴胺、3,4-二羟基苯乙酸（DOPAC）、组胺、抗坏血酸、去甲肾上腺素、腺苷和 pH 值的高灵敏度和选择性。使用 N-MCSWV，结合 PEDOT：Nafion 涂层碳纤维微电极，在乌拉坦麻醉大鼠的黑质网状部中确定了 52 ± 5.8 nM（= 20 只大鼠，±SEM）的紧张型 5-羟色胺浓度。多巴胺能、去甲肾上腺素能和 5-羟色胺能突触再摄取抑制剂的药理学挑战支持了 N-MCSWV 选择性检测紧张型 5-羟色胺水平的能力。总的来说，N-MCSWV 是一种用于分析定量检测 5-羟色胺的新型伏安技术。它提供了对大脑中紧张型 5-羟色胺浓度变化的连续监测，以进一步了解 5-羟色胺在正常行为和精神障碍中的作用。
