尼日利亚学龄前儿童龋齿与非龋齿者牙菌斑中变异链球菌和远缘链球菌的对比分析。

Comparative Analysis of Streptococcus Mutans and Streptococcus Sobrinus from Dental Plaque Samples of Nigerian Pre-school Children with and Without Caries.

机构信息

Department of Child Dental Health, Lagos University Teaching Hospital, Lagos, Nigeria.

Medical Microbiology Unit, Department of Medical Laboratory Science, College of Medicine, University of Lagos.

出版信息

West Afr J Med. 2021 Oct 29;Vol. 38(10):972-978.

PMID:34855424

Abstract

BACKGROUND

Early childhood caries (ECC), despite being a preventable disease, remains prevalent in sub-Saharan Africa. This study compared the occurrence of Streptococcus mutans and Streptococcus sobrinus in dental plaque from caries free and caries active Nigerian preschool children.

METHODS

Structured questionnaire were administered to eighty (80) mothers to inquire about the sociodemographic characteristics of their children. Diagnosis of dental caries was made according to the diagnostic criteria of the World Health Organization (WHO). Supragingival plaque sample collected from caries free children and children with dental caries (cavitated and non-cavitated lesions) using wooden toothpicks were cultured anaerobically. Samples were cultured in anaerobiosis and isolates obtained were identified based on molecular characterization performed by polymerase chain reaction (PCR) analysis. Statistical package for the social sciences (SPSS 25.0) was used for analysis.

RESULTS

Streptococcus mutans was detected in 26(65.0%) children with early childhood caries, and S. sobrinus in 10(25.0%) children with early childhood caries. The presence of S. mutans and S. sobrinus occurring together was associated with a higher deft score. Occurrence of S. mutans did not show significant difference between children with ECC and caries free children (p=0.099), however, the proportion of children with ECC positive for S. sobrinus was significantly higher than that of caries free children (p= 0.003).

CONCLUSIONS

S. mutans and S. sobrinus had a similar frequency of occurrence in children with ECC and caries free children. The occurrence of S. mutans alongside S. sobrinus in ECC is related to its severity.

摘要

背景

尽管婴幼儿龋（ECC）是一种可预防的疾病，但在撒哈拉以南非洲仍然普遍存在。本研究比较了无龋和龋活跃的尼日利亚学龄前儿童牙菌斑中变形链球菌和远缘链球菌的发生情况。

方法

对 80 名母亲进行了结构化问卷调查，以了解其子女的社会人口统计学特征。根据世界卫生组织（WHO）的诊断标准进行龋齿诊断。使用木制牙签从无龋儿童和有龋齿（龋洞和非龋洞病变）的儿童中采集龈上菌斑样本，在厌氧条件下进行培养。样本在厌氧条件下培养，根据聚合酶链反应（PCR）分析进行的分子特征鉴定获得的分离株。使用社会科学统计软件包（SPSS 25.0）进行分析。

结果

在 26 名（65.0%）患有早期儿童龋的儿童中检测到变形链球菌，在 10 名（25.0%）患有早期儿童龋的儿童中检测到远缘链球菌。S. mutans 和 S. sobrinus 同时存在与更高的 deft 评分相关。ECC 儿童和无龋儿童的 S. mutans 存在无显著差异（p=0.099），但 ECC 儿童中 S. sobrinus 的阳性率明显高于无龋儿童（p=0.003）。