Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA.
UGA Laboratorio Nacional de Genómica para la Biodiversidad, CINVESTAV Irapuato, Guanajuato 36821, México.
Science. 2021 Dec 3;374(6572):1247-1252. doi: 10.1126/science.abj2327. Epub 2021 Dec 2.
Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of . Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher-order mutants in both maize and have reduced numbers of cortical layers, showing that the pathway controls expansion of cortical tissue to elaborate anatomical complexity.
大多数植物根具有多个皮层层，这些皮层层构成了器官的大部分，并在生理学中发挥关键作用，例如耐洪水和共生。然而，对于除了高度简化的 结构之外的皮层层的形成知之甚少。在这里，我们使用单细胞 RNA 测序快速生成了玉米根的细胞分辨率图谱，揭示了组织形成转录因子短根（SHORT-ROOT，SHR）与扩展皮层相邻的替代构型。我们表明，玉米 SHR 蛋白具有高度流动性，至少移动八个细胞层进入皮层。玉米和 中的高次 突变体皮层层数量减少，表明 途径控制皮层组织的扩张以精细构建解剖结构的复杂性。
