Sasaki Y, Tuberville A W, Wood T O, McLaughlin B J
Invest Ophthalmol Vis Sci. 1986 Apr;27(4):480-5.
Intramembrane changes occurring in dysfunctional corneal endothelial cell membranes were examined using freeze-fracture and transmission electron microscopy techniques. Three categories of dysfunctional endothelium were examined: aphakic bullous keratopathy, pseudophakic bullous keratopathy and Fuchs' endothelial dystrophy. Keratoconus corneas and a donor eye bank eye were examined as normal controls. Four intramembrane changes were observed on replicas of freeze-fractured membranes in each category of dysfunctional endothelium. These were a marked reduction in intramembrane particle density on lateral membranes, altered apico-lateral junctional complexes, increased vesicle fusion sites on apical, lateral and basal membranes, and abnormal desmosome-like particle aggregates on the lateral endothelial cell membranes. The marked reduction in intramembrane particles on lateral membranes may be due to a change in the macromolecular components associated with pump dysfunction. The increase in membrane vesicle fusion sites and the breakdown in intercellular junctions may be associated with increased permeability during barrier dysfunction.
利用冷冻蚀刻和透射电子显微镜技术，对功能失调的角膜内皮细胞膜中发生的膜内变化进行了研究。研究了三类功能失调的内皮：无晶状体大泡性角膜病变、人工晶状体大泡性角膜病变和富克斯内皮营养不良。圆锥角膜和供体眼库眼作为正常对照进行了检查。在每类功能失调的内皮的冷冻蚀刻膜复制品上观察到四种膜内变化。这些变化包括侧膜上膜内颗粒密度显著降低、顶-侧连接复合体改变、顶膜、侧膜和基底膜上囊泡融合位点增加以及侧内皮细胞膜上出现异常的桥粒样颗粒聚集。侧膜上膜内颗粒的显著减少可能是由于与泵功能障碍相关的大分子成分的变化。膜囊泡融合位点的增加和细胞间连接的破坏可能与屏障功能障碍期间通透性增加有关。
