Zhu Zheng, Liao Lvyan, Su Jiawen, Liu Zhenni, Pan Shulei, Huang Yifan, Wu Yijian
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China; South China Agriculture University, College Marine Science, Guangzhou, 510642, Guangdong, People's Republic of China.
Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture And Forestry University, Fuzhou, 350002, People's Republic of China; University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fuzhou, 350002, People's Republic of China.
Vet Microbiol. 2022 Jan;264:109286. doi: 10.1016/j.vetmic.2021.109286. Epub 2021 Nov 23.
It has been shown that Muscovy duck reovirus (MDRV) infection causes severe intestinal barrier damage and intestinal mucosal immune suppression. The health and balance of gut microbes is essential for the progression of intestinal infectious diseases. To investigate the interaction of MDRV, intestinal bacteria with host intestinal innate immunity, an MDRV contact-infection model was established in this study. High-throughput sequencing technology was used to sequence 16S rDNA and transcripts in ileal samples from experimental Muscovy ducklings. Our results suggest that intestinal opportunistic pathogens such as Streptococcus and Corynebacterium proliferated massively in MDRV-infected Muscovy ducklings. The body initiates antiviral and antibacterial immunity and actively fights the infection of gut microbes. The synthesis of peptidoglycan, lipopolysaccharide, and flagellin by intestinal bacteria activates the Toll-like receptor signaling pathway resulting in increased secretion of IFN-β, IL-1β, and IL-8. The RIG-I-like receptor signaling pathway is an important signaling pathway for the interaction between MDRV and the host. At the same time, we also observed that multiple genes in the JAK-STAT signaling pathway were significantly different. These genes are important targets for studying the immunosuppression caused by MDRV. In conclusion, we analyzed the interaction of MDRV, intestinal flora and host immune system during MDRV infection, which provides a basis for the further study on the mechanism of intestinal immunosuppression caused by MDRV.
已表明番鸭呼肠孤病毒（MDRV）感染会导致严重的肠道屏障损伤和肠道黏膜免疫抑制。肠道微生物的健康与平衡对于肠道传染病的进展至关重要。为了研究MDRV、肠道细菌与宿主肠道固有免疫之间的相互作用，本研究建立了MDRV接触感染模型。利用高通量测序技术对实验番鸭雏鸡回肠样本中的16S rDNA和转录本进行测序。我们的结果表明，在感染MDRV的番鸭雏鸡中，链球菌和棒状杆菌等肠道机会致病菌大量增殖。机体启动抗病毒和抗菌免疫，积极对抗肠道微生物的感染。肠道细菌合成的肽聚糖、脂多糖和鞭毛蛋白激活Toll样受体信号通路，导致IFN-β、IL-1β和IL-8的分泌增加。RIG-I样受体信号通路是MDRV与宿主相互作用的重要信号通路。同时，我们还观察到JAK-STAT信号通路中的多个基因存在显著差异。这些基因是研究MDRV引起的免疫抑制的重要靶点。总之，我们分析了MDRV感染过程中MDRV、肠道菌群与宿主免疫系统之间的相互作用，为进一步研究MDRV引起肠道免疫抑制的机制提供了依据。
