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Palmae 基因组中转座元件的全基因组比较分析。

Genome-wide comparative analysis of transposable elements in Palmae genomes.

机构信息

National Centre for Bioinformatics, King Abdulaziz City for Science and Technology, 11442 Riyadh, Saudi Arabia.

Centre for Biodiversity Genomics, University of Guelph, Guelph, ON N1G 2W1, Canada.

出版信息

Front Biosci (Landmark Ed). 2021 Nov 30;26(11):1119-1131. doi: 10.52586/5014.

Abstract

: Transposable elements (TEs) are the largest component of the genetic material of most eukaryotes and can play roles in shaping genome architecture and regulating phenotypic variation; thus, understanding genome evolution is only possible if we comprehend the contributions of TEs. However, the quantitative and qualitative contributions of TEs can vary, even between closely related lineages. For palm species, in particular, the dynamics of the process through which TEs have differently shaped their genomes remains poorly understood because of a lack of comparative studies. : We conducted a genome-wide comparative analysis of palm TEs, focusing on identifying and classifying TEs using the draft assemblies of four palm species: , , , and . Our TE library was generated using both structure-based and homology-based methodologies. : The generated libraries revealed the TE component of each assembly, which varied from 41-81%. Class I retrotransposons covered 36-75% of these species' draft genome sequences and primarily consisted of LTR retroelements, while non-LTR elements covered about 0.56-2.31% of each assembly, mainly as LINEs. The least represented were Class DNA transposons, comprising 1.87-3.37%. : The current study contributes to a detailed identification and characterization of transposable elements in Palmae draft genome assemblies.

摘要

转座元件 (TEs) 是大多数真核生物遗传物质的最大组成部分,可在塑造基因组结构和调节表型变异方面发挥作用;因此,如果我们要理解基因组的进化,就必须理解 TEs 的贡献。然而,即使在密切相关的谱系之间,TEs 的定量和定性贡献也可能有所不同。特别是对于棕榈物种,由于缺乏比较研究,TE 如何以不同的方式塑造其基因组的过程的动态仍然知之甚少。

我们对棕榈科 TEs 进行了全基因组比较分析,重点是使用四种棕榈物种的草案组装来识别和分类 TEs:、、、和。我们的 TE 文库使用基于结构和基于同源性的方法生成。

生成的文库揭示了每个组装的 TE 组成部分,其范围从 41-81%。I 类反转录转座子覆盖了这些物种草案基因组序列的 36-75%,主要由 LTR 反转元件组成,而非 LTR 元件约占每个组装的 0.56-2.31%,主要为 LINEs。最少的是 I 类 DNA 转座子,占 1.87-3.37%。

本研究有助于详细识别和表征 Palmae 草案基因组组装中的转座元件。

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