Campbell M, Bieber M, Levy R, Teng N N
J Immunol. 1986 Apr 15;136(8):2983-8.
Immunoglobulin (Ig) was obtained from the tumor cells of patients with B cell malignancies by somatic cell hybridization to mouse-human heteromyeloma cells. The human Ig secreted by one of these hybridomas was used as an immunogen for the production of rat monoclonal antibodies (mAb). A panel of mAb specific for the idiotype (Id) was produced and characterized. Competitive binding studies that made use of [Se]-labeled anti-Id mAb (MAID) demonstrated several distinct yet topographically related Id on the Id-bearing Ig. These antibodies were shown to have avidities ranging from 0.38 to 45.3 X 10(8) l/mol. Additional studies demonstrated varying degrees of antigenic modulation of surface Id in vitro by MAID. The degree of modulation correlates with antibody avidity.
通过将B细胞恶性肿瘤患者的肿瘤细胞与小鼠 - 人异源骨髓瘤细胞进行体细胞杂交获得免疫球蛋白（Ig）。其中一种杂交瘤分泌的人Ig被用作免疫原，用于制备大鼠单克隆抗体（mAb）。制备并鉴定了一组针对独特型（Id）的单克隆抗体。利用[Se]标记的抗独特型单克隆抗体（MAID）进行的竞争性结合研究表明，携带Id的Ig上存在几种不同但在拓扑结构上相关的Id。这些抗体的亲和力范围为0.38至45.3×10⁸ l/mol。进一步的研究表明，MAID在体外对表面Id具有不同程度的抗原调节作用。调节程度与抗体亲和力相关。
