Mattern T, Reich C, Duchrow M, Ansorge S, Ulmer A J, Flad H D
Forschungsinstitut Borstel, Department of Immunology and Cell Biology, Borstel, Germany.
Immunology. 1995 Apr;84(4):595-600.
The ability of different anti-CD26 monoclonal antibodies to modulate the expression of CD26 on human T lymphocytes was investigated. By means of a new non-radioactive method using fluorescein isothiocyanate (FITC)-labelled and unlabelled anti-CD26 monoclonal antibodies and flow cytometry, we measured the internalization and re-expression of CD26 on freshly isolated resting human T lymphocytes. The modulation of CD26 surface expression takes place in primarily CD26+ as well as in CD26- T lymphocytes, indicating the presence of an intracellular CD26 pool. In fact, with two different anti-CD26 monoclonal antibodies (Ta1 and M5) intracellular CD26 was detected out of which newly expressed CD26 might have originated. This intracellular CD26 pool appears to be maintained by continuous translation of CD26 mRNA.
研究了不同抗CD26单克隆抗体调节人T淋巴细胞上CD26表达的能力。通过一种新的非放射性方法，使用异硫氰酸荧光素（FITC）标记和未标记的抗CD26单克隆抗体以及流式细胞术，我们测量了新鲜分离的静息人T淋巴细胞上CD26的内化和重新表达。CD26表面表达的调节主要发生在CD26 +以及CD26 - T淋巴细胞中，表明存在细胞内CD26池。事实上，用两种不同的抗CD26单克隆抗体（Ta1和M5）检测到了细胞内CD26，新表达的CD26可能由此产生。这个细胞内CD26池似乎通过CD26 mRNA的持续翻译得以维持。
