McMichael A J, Michie C A, Gotch F M, Smith G L, Moss B
J Gen Virol. 1986 Apr;67 ( Pt 4):719-26. doi: 10.1099/0022-1317-67-4-719.
A recombinant vaccinia virus (NP-VAC) containing cDNA corresponding to segment 5, the nucleoprotein (NP) gene of influenza A/PR/8/34 virus was used to examine the specificity of human influenza virus immune cytotoxic T lymphocytes (CTL). Effector cell preparations from two donors recognized autologous lymphocytes that had been infected with NP-VAC. Lysis was specific because cells infected with vaccinia virus were not killed and recognition was HLA-restricted. In one donor, the influenza virus-specific CTL response changed with time so that his effector cells no longer recognized autologous lymphocytes infected with NP-VAC. However, a component that was NP-specific remained because these CTL lysed the more sensitive autologous B lymphoblastoid cells that had been infected with NP-VAC. In four other donors, no NP-specific CTL response could be detected using autologous lymphocyte targets. Thus NP, an internal virus protein, is one antigen that is recognized by human influenza A virus-specific CTL, but it is likely that other individual virus components contribute to the total CTL response.
一种重组痘苗病毒（NP-VAC），其包含与甲型流感病毒A/PR/8/34的第5节段核蛋白（NP）基因相对应的cDNA，被用于检测人流感病毒免疫细胞毒性T淋巴细胞（CTL）的特异性。来自两名供体的效应细胞制剂识别了已被NP-VAC感染的自体淋巴细胞。细胞裂解具有特异性，因为被痘苗病毒感染的细胞未被杀死，且识别具有HLA限制性。在一名供体中，流感病毒特异性CTL反应随时间变化，以至于他的效应细胞不再识别被NP-VAC感染的自体淋巴细胞。然而，一个NP特异性成分仍然存在，因为这些CTL裂解了更敏感的、已被NP-VAC感染的自体B淋巴母细胞。在其他四名供体中，使用自体淋巴细胞靶标未检测到NP特异性CTL反应。因此，NP，一种病毒内部蛋白，是被甲型人流感病毒特异性CTL识别的一种抗原，但很可能其他个别病毒成分也对总的CTL反应有贡献。
