School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, PR China.
School of Environmental Science, Nanjing XiaoZhuang University, Nanjing, 211171, PR China.
Talanta. 2022 Feb 1;238(Pt 1):123026. doi: 10.1016/j.talanta.2021.123026. Epub 2021 Nov 3.
Herein we designed a highly sensitive and selective biosensor for methamphetamine (METH) detection based on aptamer recognition probe and atom transfer radical polymerization (ATRP) signal amplification strategy. In this experiment, METH aptamer and its complementary DNA strand were first attached to the electrode surface. In the presence of METH, the prioritized conjugation between METH and the aptamer will take one strand of DNA from the double-stranded DNA, so that the third segment of azide-modified DNA could be successfully modified onto the electrode surface. Through click chemistry and ATRP polymerization, the monomers with ferrocene were polymerized into a long chain, and the signal was amplified, then high-sensitivity detection of METH can be carried out. The result showed that the sensor could detect METH as low as 17 fM, which is about two orders of magnitude lower than that by traditional METH detection methods. Moreover, when different concentrations of METH were added to serum and urine, the recovery rate of the biosensor was as high as 93%. Therefore, using nucleic acid aptamer as capture probe and ATRP as signal amplification strategy can provide a promising application platform for sensitive detection of low concentration toxicants.
在此，我们设计了一种基于适体识别探针和原子转移自由基聚合（ATRP）信号放大策略的高灵敏度和选择性检测甲基苯丙胺（METH）的生物传感器。在该实验中，METH 适体及其互补 DNA 链首先被连接到电极表面。在 METH 存在的情况下，METH 与适体之间的优先结合将从双链 DNA 中取出一条 DNA 链，从而使叠氮修饰的 DNA 的第三段能够成功地修饰到电极表面上。通过点击化学和 ATRP 聚合，将带有二茂铁的单体聚合成长链，从而放大信号，然后可以进行 METH 的高灵敏度检测。结果表明，该传感器可以检测到低至 17 fM 的 METH，比传统的 METH 检测方法低两个数量级。此外，当向血清和尿液中加入不同浓度的 METH 时，生物传感器的回收率高达 93%。因此，使用核酸适体作为捕获探针和 ATRP 作为信号放大策略可为低浓度毒物的灵敏检测提供有前途的应用平台。
