Du Jianting, Qian Jiekun, Zheng Bin, Xu Guobing, Chen Hao, Chen Chun
Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian, People's Republic of China.
Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fujian Province University, Fuzhou, Fujian, People's Republic of China.
Int J Gen Med. 2021 Nov 25;14:8873-8880. doi: 10.2147/IJGM.S337149. eCollection 2021.
Lung cancer (LUCA) is one of the most prevalent human malignancies, and the leading cause of cancer-related deaths worldwide. Previous reports have shown that miR-21-5p plays a vital role in development of various tumors. Here, we explored the relationship between miR-21-5p/PIK3R1 axis and prognosis of patients with lung adenocarcinoma (LUAD).
MiRNAseq data, deposited in The Cancer Genome Atlas (TCGA) database, was downloaded and used to determine patterns of miR-21-5p expression in both LUAD and normal lung tissues. Statistical analyses and data visualization were performed using dbDEMC v3.0 platform, starBase v3.0 database and packages implemented in R software. Next, we employed TargetScan Human, miRDB and DIANA Tools databases to predict miR-21-5p target genes, then analyzed their expression patterns as well as prognostic value in LUAD.
Most human cancers overexpressed miR-21-5p. Specifically, miR-21-5p was significantly upregulated in LUAD tissues relative to normal lung tissues (P < 0.001), and this high expression was significantly correlated with poor patient prognosis (hazard ratio [HR]=1.45, P = 0.014). PIK3R1 was predicted as a miR-21-5p target gene, and both were negatively correlated (r=-0.218, P < 0.01). Notably, PIK3R1 was significantly downregulated in LUAD, relative to normal lung tissues (P < 0.01), with its overexpression significantly associated with poor prognosis of LUAD patients (HR = 0.62, P = 0.0014).
miR-21-5p is a potential prognostic biomarker for LUAD patients. Moreover, it might be playing a role in LUAD progression by regulating PIK3R1 expression.
肺癌是人类最常见的恶性肿瘤之一，也是全球癌症相关死亡的主要原因。既往报道显示，miR-21-5p在多种肿瘤的发生发展中起重要作用。在此，我们探讨了miR-21-5p/PIK3R1轴与肺腺癌（LUAD）患者预后的关系。
下载存于癌症基因组图谱（TCGA）数据库中的miRNA测序数据，用于确定LUAD组织和正常肺组织中miR-21-5p的表达模式。使用dbDEMC v3.0平台、starBase v3.0数据库及R软件中实现的软件包进行统计分析和数据可视化。接下来，我们利用TargetScan Human、miRDB和DIANA Tools数据库预测miR-21-5p的靶基因，然后分析它们在LUAD中的表达模式及预后价值。
大多数人类癌症中miR-21-5p过表达。具体而言，相对于正常肺组织，LUAD组织中miR-21-5p显著上调（P < 0.001），且这种高表达与患者预后不良显著相关（风险比[HR]=1.45，P = 0.014）。PIK3R1被预测为miR-21-5p的靶基因，二者呈负相关（r=-0.218，P < 0.01）。值得注意的是，相对于正常肺组织，LUAD中PIK3R1显著下调（P < 0.01），其过表达与LUAD患者预后不良显著相关（HR = 0.62，P = 0.0014）。
miR-21-5p是LUAD患者潜在的预后生物标志物。此外，它可能通过调节PIK3R1的表达在LUAD进展中发挥作用。
