Liang Peng, Shang Lindong, Wang Yuntong, Booth Martin J, Li Bei
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Changchun, 130033, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Biomed Opt Express. 2021 Oct 19;12(11):7024-7032. doi: 10.1364/BOE.439054. eCollection 2021 Nov 1.
Beam shaping techniques have been widely used in holographic optical tweezers to accurately manipulate tiny particles and hologram optimization algorithms have also been widely reported to improve the optical trapping performance. In this paper, we presented a beam shaping laser induced forward transfer (BS-LIFT) technique to isolate complex-shaped cells. To do this, we built up a BS-LIFT instrument which combined beam shaping methods and laser induced forward transfer using liquid-crystal-on-silicon spatial light modulator. The laser beam was modulated into multiple desired points at the focal plane employing the Gerchberg-Saxton (GS) algorithm. Feasibility was verified through transferring various samples. To our knowledge, this is the first demonstration of BS-LIFT applied to the transfer complex-shaped cells. We successfully transferred cells whose size ranged from 1 µm to 100 µm. Our design will provide a novel approach for the application of this beam shaping technique and the isolation of single cells with variable shapes.
光束整形技术已在全息光镊中广泛应用，用于精确操纵微小粒子，并且全息图优化算法也被广泛报道以改善光阱性能。在本文中，我们提出了一种光束整形激光诱导正向转移（BS-LIFT）技术来分离复杂形状的细胞。为此，我们构建了一台BS-LIFT仪器，该仪器结合了光束整形方法和使用硅基液晶空间光调制器的激光诱导正向转移。利用格尔奇贝格-萨克斯顿（GS）算法将激光束在焦平面上调制为多个所需的点。通过转移各种样品验证了可行性。据我们所知，这是首次证明BS-LIFT应用于转移复杂形状的细胞。我们成功转移了尺寸范围从1微米到100微米的细胞。我们的设计将为这种光束整形技术的应用以及分离具有可变形状的单细胞提供一种新方法。
