Emergency Department, Weifang People's Hospital, Weifang, 261041, China.
Breast Diagnosis and Treatment Center, Affiliated Qingdao Central Hospital, Qingdao University, Qingdao, 266042, China.
Clin Transl Oncol. 2022 May;24(5):882-891. doi: 10.1007/s12094-021-02736-0. Epub 2021 Dec 3.
Breast cancer (BC) is one of the most common malignant tumors for women. The role and potential mechanisms of long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) were explored in BC cell migration and invasion.
PVT1, miR-148a-3p and Rho‑associated, coiled‑coil containing protein kinase 1 (ROCK1) mRNA expressions were detected using real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). The ROCK1 protein expression was detected by Western blotting. The relationship of PVT1, miR-148a-3p and ROCK1 was analyzed by Dual Luciferase activity, RNA immunoprecipitation (RIP) and Spearman correlation analysis. Cell invasion and migration were detected by Transwell assay.
Upregulation of PVT1 and ROCK1, and downregulation of miR-148a-3p were observed in BC tissues and cell lines. According to the analysis of Dual Luciferase activity, RIP and Spearman correlation analysis, miR-148a-3p directly binds to PVT1, and ROCK1 is a target of miR-148a-3p. In addition, PVT1 regulated the cells migration and invasion by regulating miR-148a-3p and ROCK1 expression.
These data demonstrated that PVT1 was upregulated and facilitated to the cell migration and invasion of BC by the regulation of miR-148a-3p and ROCK1, indicating that PVT1 may be a potential biomarker of BC diagnosis and treatment.
乳腺癌（BC）是女性最常见的恶性肿瘤之一。本研究旨在探讨长链非编码 RNA 浆细胞瘤变异易位 1（lncRNA PVT1）在 BC 细胞迁移和侵袭中的作用及其潜在机制。
采用实时荧光定量聚合酶链反应（qRT-PCR）检测 PVT1、miR-148a-3p 和 Rho 相关卷曲螺旋蛋白激酶 1（ROCK1）mRNA 的表达。采用 Western blot 检测 ROCK1 蛋白的表达。通过双荧光素酶活性分析、RNA 免疫沉淀（RIP）和 Spearman 相关性分析来分析 PVT1、miR-148a-3p 和 ROCK1 之间的关系。通过 Transwell 实验检测细胞侵袭和迁移。
BC 组织和细胞系中观察到 PVT1 和 ROCK1 上调，miR-148a-3p 下调。通过双荧光素酶活性分析、RIP 和 Spearman 相关性分析发现，miR-148a-3p 可直接与 PVT1 结合，ROCK1 是 miR-148a-3p 的靶基因。此外，PVT1 通过调节 miR-148a-3p 和 ROCK1 的表达来调节细胞迁移和侵袭。
这些数据表明，PVT1 通过调节 miR-148a-3p 和 ROCK1 的表达而上调，并促进 BC 细胞的迁移和侵袭，表明 PVT1 可能是 BC 诊断和治疗的潜在生物标志物。
