Ke Xia, Shen Yi, Cao Lisha, Zhang Bo, Liu Zhiqiang
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Nov 25;37(11):3975-3987. doi: 10.13345/j.cjb.200772.
Sterols, a class of cyclopentane poly-hydrophenanthrene derivatives, are the predominant membrane constituent of eukaryotes. These substances have a variety of biological activities and have been widely used in food and pharmaceutical industries. The presence of endogenous ergosterol biosynthetic pathway in Saccharomyces cerevisiae cells make it an ideal chassis for the de novo synthesis of sterol and its derivatives. Most recently, the rational modification of organelles provides a novel strategy for the directed transportation and storage of target products and the ultimate enhanced product synthesis. This review summarizes the phenotypic responses of S. cerevisiae cells upon different physiological stimulations and the underlying molecular mechanisms. Reinforcement of sterol production through directed storage, transportation, and excretion of sterols offers a novel strategy for breaking the limitation of de novo biosynthesis of sterols in yeast.
甾醇是一类环戊烷多氢菲衍生物，是真核生物膜的主要成分。这些物质具有多种生物活性，已广泛应用于食品和制药行业。酿酒酵母细胞内源性麦角固醇生物合成途径的存在，使其成为从头合成甾醇及其衍生物的理想底盘。最近，细胞器的合理修饰为目标产物的定向运输和储存以及最终提高产物合成提供了一种新策略。本文综述了酿酒酵母细胞在不同生理刺激下的表型反应及其潜在的分子机制。通过甾醇的定向储存、运输和排泄来加强甾醇的生产，为突破酵母中甾醇从头生物合成的限制提供了一种新策略。
