Saullo Carolina M, Sinzato Yuri K, Paula Verônyca G, Gallego Franciane Q, Corrente José E, Iessi Isabela L, Volpato Gustavo T, Damasceno Débora C
Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate Course on Tocogynecology, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, Sao Paulo, Brazil.
Research Support Office, Botucatu Medical School, Sao Paulo State University (Unesp), Botucatu, Sao Paulo, Brazil.
J Dev Orig Health Dis. 2022 Oct;13(5):634-641. doi: 10.1017/S2040174421000672. Epub 2021 Dec 3.
Clinical and epidemiological studies show that maternal hyperglycemia can change the programming of offspring leading to transgenerational effects. These changes may be related to environmental factors, such as high-fat diet (HFD) consumption, and contribute to the comorbidity onset at the adulthood of the offspring. The objective of this study was to evaluate the hyperglycemic intrauterine environment, associated or not with an HFD administered from weaning to adult life on the periovarian adipose tissue of rat offspring Maternal diabetes was chemically induced by Streptozotocin. Female offsprings were randomly distributed into four experimental groups (n = 5 animals/group): Female offspring from control or diabetic mothers and fed an HFD or standard diet. HFD was prepared with lard enrichment and given from weaning to adulthood. On day 120 of life, the rats were anesthetized and sacrificed to obtain adipose tissue samples. Then, the hyperglycemic intrauterine environment and HFD fed after weaning caused a higher body weight, total fat, and periovarian fat in adult offspring, which could compromise the future reproductive function of these females. These rats showed higher adiposity index and adipocyte area, contributing to hypertrophied adipose tissue. Therefore, maternal diabetes itself causes intergenerational changes and, in association with the HFD consumption after weaning, exacerbated the changes in the adipose tissue of adult female offspring.
临床和流行病学研究表明，母体高血糖会改变后代的编程，导致跨代效应。这些变化可能与环境因素有关，如高脂饮食（HFD）摄入，并导致后代成年期合并症的发生。本研究的目的是评估高血糖宫内环境，无论是否与从断奶到成年期给予的高脂饮食有关，对大鼠后代卵巢周围脂肪组织的影响。通过链脲佐菌素化学诱导母体糖尿病。雌性后代被随机分为四个实验组（每组n = 5只动物）：来自对照或糖尿病母亲的雌性后代，并喂食高脂饮食或标准饮食。高脂饮食用猪油强化制备，从断奶到成年期给予。在生命的第120天，将大鼠麻醉并处死以获得脂肪组织样本。然后，高血糖宫内环境和断奶后喂食高脂饮食导致成年后代体重、总脂肪和卵巢周围脂肪增加，这可能会损害这些雌性动物未来的生殖功能。这些大鼠显示出更高的肥胖指数和脂肪细胞面积，导致脂肪组织肥大。因此，母体糖尿病本身会引起代际变化，并且与断奶后高脂饮食的摄入相关，加剧了成年雌性后代脂肪组织的变化。
