用于尿液检测的11-去甲-Δ⁹-四氢大麻酚羧酸的分散液液微萃取

Dispersive liquid-liquid microextraction of 11-nor-Δ9-tetrahydrocannabinol-carboxylic acid applied to urine testing.

作者信息

Rodrigues Leonardo C, Kahl Júlia Mm, de Chinaglia Kauê O, de Campos Eduardo G, Costa José Luiz

机构信息

Campinas Poison Control Center, Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, 13083-859, Brazil.

Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, São Paulo, 13083-859, Brazil.

出版信息

Bioanalysis. 2022 Jan;14(2):87-100. doi: 10.4155/bio-2021-0237.

DOI:10.4155/bio-2021-0237

PMID:34860122

Abstract

THC-COOH is the major metabolite of Δ-tetrahydrocannabinol commonly tested in urine to determine cannabis intake. In this study, a method based on dispersive liquid-liquid microextraction was developed for testing THC-COOH in urine. Hydrolyzed urine specimens were extracted via dispersive liquid-liquid microextraction with acetonitrile (disperser solvent) and chloroform (extraction solvent). Derivatization was performed with -Bis(trimethylsilyl)trifluoroacetamide with 1% trichloro(chloromethyl)silane. Analysis was performed by GC-MS/MS. The method showed acceptable linearity (5-500 ng/ml), imprecision (<10.5%) and bias (<4.9%). Limits of detection and quantitation were 1 and 5 ng/ml, respectively. Twenty-four authentic samples were analyzed, with 22 samples being positive for THC-COOH. The proposed method is more environmentally friendly and provided good sensitivity, selectivity and reproducibility.

摘要

四氢大麻酚酸（THC - COOH）是尿液中常检测的Δ-四氢大麻酚的主要代谢产物，用于确定大麻摄入量。本研究开发了一种基于分散液液微萃取的方法来检测尿液中的THC - COOH。水解后的尿液标本通过用乙腈（分散剂溶剂）和氯仿（萃取溶剂）进行分散液液微萃取来提取。用含1%三氯（氯甲基）硅烷的双（三甲基硅基）三氟乙酰胺进行衍生化。通过气相色谱 - 串联质谱法进行分析。该方法显示出可接受的线性（5 - 500 ng/ml）、不精密度（<10.5%）和偏差（<4.9%）。检测限和定量限分别为1和5 ng/ml。分析了24个真实样本，其中22个样本的THC - COOH呈阳性。所提出的方法更环保，并且具有良好的灵敏度、选择性和重现性。