一种从阳性血培养物中直接进行细菌鉴定和抗菌药物敏感性测试的快速方法。
A rapid procedure for bacterial identification and antimicrobial susceptibility testing directly from positive blood cultures.
机构信息
Institute of Medical Photonics, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
出版信息
Analyst. 2021 Dec 20;147(1):147-154. doi: 10.1039/d1an01210j.
There is an urgent need to develop a rapid procedure that can rapidly identify and obtain antimicrobial susceptibility testing (AST) results directly from positive blood cultures. Here, we report a semi-automatic bacterial diagnosis procedure, which includes (1) a bacterial concentration process to isolate bacteria from a positive blood culture bottle (PBCB), (2) an identification process using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and (3) a rapid AST process based on stimulated Raman scattering imaging of deuterium oxide (DO) incorporation in bacteria. A total of 105 samples were tested for bacterial identification, and a bacterial identification accuracy of 92.3% was achieved. AST takes about 2.5 h after identification. This semi-automatic procedure only takes 3.5 h, which is demonstrated to be the fastest process to obtain identification and AST results starting from PBCBs.
目前迫切需要开发一种快速程序，能够直接从阳性血培养瓶中快速鉴定并获得抗菌药物敏感性检测（AST）结果。在这里，我们报告了一种半自动细菌诊断程序，包括（1）细菌浓缩过程，从阳性血培养瓶（PBCB）中分离细菌，（2）使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行鉴定的过程，以及（3）基于细菌中氘氧化物（DO）掺入的受激拉曼散射成像的快速 AST 过程。总共测试了 105 个样本进行细菌鉴定，细菌鉴定的准确率达到了 92.3%。AST 大约在鉴定后 2.5 小时进行。该半自动程序仅需 3.5 小时，从 PBCB 获得鉴定和 AST 结果的速度是最快的。
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